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Genomic characteristics of an avipoxvirus 282E4 strain

Avipoxvirus 282E4 strain was extensively applied into recombinant vaccine vector to prevent other infectious diseases. However, little information on the genomic background, functional and genetic evolutionary of the isolate 282E4 strain was clarified. The results showed that the linear genome of av...

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Autores principales: Deng, Lingcong, Liu, Cunxia, Li, Letian, Hao, Pengfei, Wang, Maopeng, Jin, Ningyi, Yin, Ronglan, Du, Shouwen, Li, Chang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507152/
https://www.ncbi.nlm.nih.gov/pubmed/37678517
http://dx.doi.org/10.1016/j.virusres.2023.199218
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author Deng, Lingcong
Liu, Cunxia
Li, Letian
Hao, Pengfei
Wang, Maopeng
Jin, Ningyi
Yin, Ronglan
Du, Shouwen
Li, Chang
author_facet Deng, Lingcong
Liu, Cunxia
Li, Letian
Hao, Pengfei
Wang, Maopeng
Jin, Ningyi
Yin, Ronglan
Du, Shouwen
Li, Chang
author_sort Deng, Lingcong
collection PubMed
description Avipoxvirus 282E4 strain was extensively applied into recombinant vaccine vector to prevent other infectious diseases. However, little information on the genomic background, functional and genetic evolutionary of the isolate 282E4 strain was clarified. The results showed that the linear genome of avipoxvirus 282E4 was 308,826 bp, containing 313 open reading frames (ORFs) and 12 new predicted ORFs. The 282E4 strain appears to encode two novel thymidine kinase proteins and two TGF-beta-like proteins that may be associated with the suppression of the host's antiviral response. Avipoxvirus 282E4 also encodes 57 ankyrin repeat proteins and 5 variola B22R-like proteins, which composed 7% of the avipoxvirus 282E4 genome. GO and KEGG analysis further revealed that 12 ORFs participate in viral transcription process, 7 ORFs may function during DNA repair, replication and biological synthesis, and ORF 208 is involved in the process of virus life cycle. Interestingly, phylogenetic analysis based on concatenated sequences p4b and DNA polymerase of avipoxviruses gene demonstrates that avipoxvirus 282E4 strain is divergent from known FWPV isolates and is similar to shearwater poxvirus (SWPV-1) that belongs to the CNPV-like virus. Sequencing avipoxvirus 282E4 is a significant step to judge the genetic position of avipoxviruses within the larger Poxviridae phylogenetic tree and provide a new insight into the genetic background of avipoxvirus 282E4 and interspecies transmission of poxviruses, meanwhile, explanation of gene function provides theoretical foundation for vaccine design with 282E4 strain as skeleton.
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spelling pubmed-105071522023-09-20 Genomic characteristics of an avipoxvirus 282E4 strain Deng, Lingcong Liu, Cunxia Li, Letian Hao, Pengfei Wang, Maopeng Jin, Ningyi Yin, Ronglan Du, Shouwen Li, Chang Virus Res Article Avipoxvirus 282E4 strain was extensively applied into recombinant vaccine vector to prevent other infectious diseases. However, little information on the genomic background, functional and genetic evolutionary of the isolate 282E4 strain was clarified. The results showed that the linear genome of avipoxvirus 282E4 was 308,826 bp, containing 313 open reading frames (ORFs) and 12 new predicted ORFs. The 282E4 strain appears to encode two novel thymidine kinase proteins and two TGF-beta-like proteins that may be associated with the suppression of the host's antiviral response. Avipoxvirus 282E4 also encodes 57 ankyrin repeat proteins and 5 variola B22R-like proteins, which composed 7% of the avipoxvirus 282E4 genome. GO and KEGG analysis further revealed that 12 ORFs participate in viral transcription process, 7 ORFs may function during DNA repair, replication and biological synthesis, and ORF 208 is involved in the process of virus life cycle. Interestingly, phylogenetic analysis based on concatenated sequences p4b and DNA polymerase of avipoxviruses gene demonstrates that avipoxvirus 282E4 strain is divergent from known FWPV isolates and is similar to shearwater poxvirus (SWPV-1) that belongs to the CNPV-like virus. Sequencing avipoxvirus 282E4 is a significant step to judge the genetic position of avipoxviruses within the larger Poxviridae phylogenetic tree and provide a new insight into the genetic background of avipoxvirus 282E4 and interspecies transmission of poxviruses, meanwhile, explanation of gene function provides theoretical foundation for vaccine design with 282E4 strain as skeleton. Elsevier 2023-09-13 /pmc/articles/PMC10507152/ /pubmed/37678517 http://dx.doi.org/10.1016/j.virusres.2023.199218 Text en © 2023 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Deng, Lingcong
Liu, Cunxia
Li, Letian
Hao, Pengfei
Wang, Maopeng
Jin, Ningyi
Yin, Ronglan
Du, Shouwen
Li, Chang
Genomic characteristics of an avipoxvirus 282E4 strain
title Genomic characteristics of an avipoxvirus 282E4 strain
title_full Genomic characteristics of an avipoxvirus 282E4 strain
title_fullStr Genomic characteristics of an avipoxvirus 282E4 strain
title_full_unstemmed Genomic characteristics of an avipoxvirus 282E4 strain
title_short Genomic characteristics of an avipoxvirus 282E4 strain
title_sort genomic characteristics of an avipoxvirus 282e4 strain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507152/
https://www.ncbi.nlm.nih.gov/pubmed/37678517
http://dx.doi.org/10.1016/j.virusres.2023.199218
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