High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesis

High-quality, straightforward single-cell RNA sequencing (RNA-seq) with spatial resolution remains challenging. Here, we developed DRaqL (direct RNA recovery and quenching for laser capture microdissection), an experimental approach for efficient cell lysis of tissue sections, directly applicable to...

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Detalles Bibliográficos
Autores principales: Ikeda, Hiroki, Miyao, Shintaro, Nagaoka, So, Takashima, Tomoya, Law, Sze-Ming, Yamamoto, Takuya, Kurimoto, Kazuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507249/
https://www.ncbi.nlm.nih.gov/pubmed/37722727
http://dx.doi.org/10.26508/lsa.202301929

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