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Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia

ABSTRACT BACKGROUND: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8(+) T cells in acute leukemia. METHODS: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP, EAT-2, and LncRNA-GSTT1-AS1 we...

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Detalles Bibliográficos
Autores principales: Mohammadi, Maryam, Asgarian-Omran, Hossein, Najafi, Ahmad, Valadan, Reza, Karami, Hossein, Naderisoraki, Mohammad, Zaboli, Ehsan, Eslami, Mohammad, Tehrani, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507292/
https://www.ncbi.nlm.nih.gov/pubmed/37634081
http://dx.doi.org/10.52547/ibj.3843
Descripción
Sumario:ABSTRACT BACKGROUND: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8(+) T cells in acute leukemia. METHODS: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP, EAT-2, and LncRNA-GSTT1-AS1 were evaluated using qRT-PCR. RESULTS: Expression of CD244, SAP, and EAT-2 were significantly lower in CD8(+) T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects. CONCLUSION: The low SAP/CD244 expression ratio in CD8(+) T cells in ALL suggests an inhibitory role for CD244 in ALL.