Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia

ABSTRACT BACKGROUND: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8(+) T cells in acute leukemia. METHODS: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP, EAT-2, and LncRNA-GSTT1-AS1 we...

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Autores principales: Mohammadi, Maryam, Asgarian-Omran, Hossein, Najafi, Ahmad, Valadan, Reza, Karami, Hossein, Naderisoraki, Mohammad, Zaboli, Ehsan, Eslami, Mohammad, Tehrani, Mohsen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pasteur Institute of Iran 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507292/
https://www.ncbi.nlm.nih.gov/pubmed/37634081
http://dx.doi.org/10.52547/ibj.3843
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author Mohammadi, Maryam
Asgarian-Omran, Hossein
Najafi, Ahmad
Valadan, Reza
Karami, Hossein
Naderisoraki, Mohammad
Zaboli, Ehsan
Eslami, Mohammad
Tehrani, Mohsen
author_facet Mohammadi, Maryam
Asgarian-Omran, Hossein
Najafi, Ahmad
Valadan, Reza
Karami, Hossein
Naderisoraki, Mohammad
Zaboli, Ehsan
Eslami, Mohammad
Tehrani, Mohsen
author_sort Mohammadi, Maryam
collection PubMed
description ABSTRACT BACKGROUND: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8(+) T cells in acute leukemia. METHODS: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP, EAT-2, and LncRNA-GSTT1-AS1 were evaluated using qRT-PCR. RESULTS: Expression of CD244, SAP, and EAT-2 were significantly lower in CD8(+) T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects. CONCLUSION: The low SAP/CD244 expression ratio in CD8(+) T cells in ALL suggests an inhibitory role for CD244 in ALL.
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spelling pubmed-105072922023-09-20 Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia Mohammadi, Maryam Asgarian-Omran, Hossein Najafi, Ahmad Valadan, Reza Karami, Hossein Naderisoraki, Mohammad Zaboli, Ehsan Eslami, Mohammad Tehrani, Mohsen Iran Biomed J Short Communication ABSTRACT BACKGROUND: This study investigated the role of the ICR, CD244, and its adapter molecules, in CD8(+) T cells in acute leukemia. METHODS: Blood samples were obtained from 21 ALL and 6 AML patients and 20 control subjects. Relative gene expression of CD244, SAP, EAT-2, and LncRNA-GSTT1-AS1 were evaluated using qRT-PCR. RESULTS: Expression of CD244, SAP, and EAT-2 were significantly lower in CD8(+) T cells from ALL patients than those from control subjects. Interestingly, the expression of SAP was much lower than that of CD244, indicating a lower ratio of SAP to CD244. Also, SAP expression was significantly lower in AML patients compared to the control group. Expression of LncRNA-GSTT1-AS1 showed no significant difference in ALL and AML patients compared to control subjects. CONCLUSION: The low SAP/CD244 expression ratio in CD8(+) T cells in ALL suggests an inhibitory role for CD244 in ALL. Pasteur Institute of Iran 2023-07 2023-06-27 /pmc/articles/PMC10507292/ /pubmed/37634081 http://dx.doi.org/10.52547/ibj.3843 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Mohammadi, Maryam
Asgarian-Omran, Hossein
Najafi, Ahmad
Valadan, Reza
Karami, Hossein
Naderisoraki, Mohammad
Zaboli, Ehsan
Eslami, Mohammad
Tehrani, Mohsen
Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title_full Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title_fullStr Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title_full_unstemmed Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title_short Evaluation of mRNA Expression of CD244 and Its Adapter Molecules in CD8(+) T Cells in Acute Leukemia
title_sort evaluation of mrna expression of cd244 and its adapter molecules in cd8(+) t cells in acute leukemia
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507292/
https://www.ncbi.nlm.nih.gov/pubmed/37634081
http://dx.doi.org/10.52547/ibj.3843
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