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Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity

Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli an...

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Autores principales: Buchmann, David, Schwabe, Michael, Weiss, Romano, Kuss, Andreas W., Schaufler, Katharina, Schlüter, Rabea, Rödiger, Stefan, Guenther, Sebastian, Schultze, Nadin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507321/
https://www.ncbi.nlm.nih.gov/pubmed/37731930
http://dx.doi.org/10.3389/fmicb.2023.1232039
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author Buchmann, David
Schwabe, Michael
Weiss, Romano
Kuss, Andreas W.
Schaufler, Katharina
Schlüter, Rabea
Rödiger, Stefan
Guenther, Sebastian
Schultze, Nadin
author_facet Buchmann, David
Schwabe, Michael
Weiss, Romano
Kuss, Andreas W.
Schaufler, Katharina
Schlüter, Rabea
Rödiger, Stefan
Guenther, Sebastian
Schultze, Nadin
author_sort Buchmann, David
collection PubMed
description Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli and cellulose formation in E. coli has been shown for different phenolic natural compounds like epigallocatechin gallate. This study demonstrates this effect for other structurally unrelated phenolics, namely octyl gallate, scutellarein and wedelolactone. To verify whether these structurally different compounds influence identical pathways of biofilm formation in E. coli a broad comparative RNA-sequencing approach was chosen with additional RT-qPCR to gain initial insights into the pathways affected at the transcriptomic level. Bioinformatical analysis of the RNA-Seq data was performed using DESeq2, BioCyc and KEGG Mapper. The comparative bioinformatics analysis on the pathways revealed that, irrespective of their structure, all compounds mainly influenced similar biological processes. These pathways included bacterial motility, chemotaxis, biofilm formation as well as metabolic processes like arginine biosynthesis and tricarboxylic acid cycle. Overall, this work provides the first insights into the potential mechanisms of action of novel phenolic biofilm inhibitors and highlights the complex regulatory processes of biofilm formation in E. coli.
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spelling pubmed-105073212023-09-20 Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity Buchmann, David Schwabe, Michael Weiss, Romano Kuss, Andreas W. Schaufler, Katharina Schlüter, Rabea Rödiger, Stefan Guenther, Sebastian Schultze, Nadin Front Microbiol Microbiology Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli and cellulose formation in E. coli has been shown for different phenolic natural compounds like epigallocatechin gallate. This study demonstrates this effect for other structurally unrelated phenolics, namely octyl gallate, scutellarein and wedelolactone. To verify whether these structurally different compounds influence identical pathways of biofilm formation in E. coli a broad comparative RNA-sequencing approach was chosen with additional RT-qPCR to gain initial insights into the pathways affected at the transcriptomic level. Bioinformatical analysis of the RNA-Seq data was performed using DESeq2, BioCyc and KEGG Mapper. The comparative bioinformatics analysis on the pathways revealed that, irrespective of their structure, all compounds mainly influenced similar biological processes. These pathways included bacterial motility, chemotaxis, biofilm formation as well as metabolic processes like arginine biosynthesis and tricarboxylic acid cycle. Overall, this work provides the first insights into the potential mechanisms of action of novel phenolic biofilm inhibitors and highlights the complex regulatory processes of biofilm formation in E. coli. Frontiers Media S.A. 2023-09-04 /pmc/articles/PMC10507321/ /pubmed/37731930 http://dx.doi.org/10.3389/fmicb.2023.1232039 Text en Copyright © 2023 Buchmann, Schwabe, Weiss, Kuss, Schaufler, Schlüter, Rödiger, Guenther and Schultze. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Buchmann, David
Schwabe, Michael
Weiss, Romano
Kuss, Andreas W.
Schaufler, Katharina
Schlüter, Rabea
Rödiger, Stefan
Guenther, Sebastian
Schultze, Nadin
Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title_full Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title_fullStr Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title_full_unstemmed Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title_short Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
title_sort natural phenolic compounds as biofilm inhibitors of multidrug-resistant escherichia coli – the role of similar biological processes despite structural diversity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507321/
https://www.ncbi.nlm.nih.gov/pubmed/37731930
http://dx.doi.org/10.3389/fmicb.2023.1232039
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