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Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity
Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli an...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507321/ https://www.ncbi.nlm.nih.gov/pubmed/37731930 http://dx.doi.org/10.3389/fmicb.2023.1232039 |
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author | Buchmann, David Schwabe, Michael Weiss, Romano Kuss, Andreas W. Schaufler, Katharina Schlüter, Rabea Rödiger, Stefan Guenther, Sebastian Schultze, Nadin |
author_facet | Buchmann, David Schwabe, Michael Weiss, Romano Kuss, Andreas W. Schaufler, Katharina Schlüter, Rabea Rödiger, Stefan Guenther, Sebastian Schultze, Nadin |
author_sort | Buchmann, David |
collection | PubMed |
description | Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli and cellulose formation in E. coli has been shown for different phenolic natural compounds like epigallocatechin gallate. This study demonstrates this effect for other structurally unrelated phenolics, namely octyl gallate, scutellarein and wedelolactone. To verify whether these structurally different compounds influence identical pathways of biofilm formation in E. coli a broad comparative RNA-sequencing approach was chosen with additional RT-qPCR to gain initial insights into the pathways affected at the transcriptomic level. Bioinformatical analysis of the RNA-Seq data was performed using DESeq2, BioCyc and KEGG Mapper. The comparative bioinformatics analysis on the pathways revealed that, irrespective of their structure, all compounds mainly influenced similar biological processes. These pathways included bacterial motility, chemotaxis, biofilm formation as well as metabolic processes like arginine biosynthesis and tricarboxylic acid cycle. Overall, this work provides the first insights into the potential mechanisms of action of novel phenolic biofilm inhibitors and highlights the complex regulatory processes of biofilm formation in E. coli. |
format | Online Article Text |
id | pubmed-10507321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105073212023-09-20 Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity Buchmann, David Schwabe, Michael Weiss, Romano Kuss, Andreas W. Schaufler, Katharina Schlüter, Rabea Rödiger, Stefan Guenther, Sebastian Schultze, Nadin Front Microbiol Microbiology Multidrug-resistant gram-negative pathogens such as Escherichia coli have become increasingly difficult to treat and therefore alternative treatment options are needed. Targeting virulence factors like biofilm formation could be one such option. Inhibition of biofilm-related structures like curli and cellulose formation in E. coli has been shown for different phenolic natural compounds like epigallocatechin gallate. This study demonstrates this effect for other structurally unrelated phenolics, namely octyl gallate, scutellarein and wedelolactone. To verify whether these structurally different compounds influence identical pathways of biofilm formation in E. coli a broad comparative RNA-sequencing approach was chosen with additional RT-qPCR to gain initial insights into the pathways affected at the transcriptomic level. Bioinformatical analysis of the RNA-Seq data was performed using DESeq2, BioCyc and KEGG Mapper. The comparative bioinformatics analysis on the pathways revealed that, irrespective of their structure, all compounds mainly influenced similar biological processes. These pathways included bacterial motility, chemotaxis, biofilm formation as well as metabolic processes like arginine biosynthesis and tricarboxylic acid cycle. Overall, this work provides the first insights into the potential mechanisms of action of novel phenolic biofilm inhibitors and highlights the complex regulatory processes of biofilm formation in E. coli. Frontiers Media S.A. 2023-09-04 /pmc/articles/PMC10507321/ /pubmed/37731930 http://dx.doi.org/10.3389/fmicb.2023.1232039 Text en Copyright © 2023 Buchmann, Schwabe, Weiss, Kuss, Schaufler, Schlüter, Rödiger, Guenther and Schultze. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Buchmann, David Schwabe, Michael Weiss, Romano Kuss, Andreas W. Schaufler, Katharina Schlüter, Rabea Rödiger, Stefan Guenther, Sebastian Schultze, Nadin Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title | Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title_full | Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title_fullStr | Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title_full_unstemmed | Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title_short | Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli – the role of similar biological processes despite structural diversity |
title_sort | natural phenolic compounds as biofilm inhibitors of multidrug-resistant escherichia coli – the role of similar biological processes despite structural diversity |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507321/ https://www.ncbi.nlm.nih.gov/pubmed/37731930 http://dx.doi.org/10.3389/fmicb.2023.1232039 |
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