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Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma

INTRODUCTION: One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-h...

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Autores principales: Wang, Lei, Zhang, Luojun, Zheng, Guangtao, Luo, Haiping, El-kott, Attalla F., El-kenawy, Ayman E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507773/
https://www.ncbi.nlm.nih.gov/pubmed/37732051
http://dx.doi.org/10.5114/aoms/138146
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author Wang, Lei
Zhang, Luojun
Zheng, Guangtao
Luo, Haiping
El-kott, Attalla F.
El-kenawy, Ayman E.
author_facet Wang, Lei
Zhang, Luojun
Zheng, Guangtao
Luo, Haiping
El-kott, Attalla F.
El-kenawy, Ayman E.
author_sort Wang, Lei
collection PubMed
description INTRODUCTION: One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions. MATERIAL AND METHODS: Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract. RESULTS: The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC(50) of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer. CONCLUSIONS: The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line.
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spelling pubmed-105077732023-09-20 Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma Wang, Lei Zhang, Luojun Zheng, Guangtao Luo, Haiping El-kott, Attalla F. El-kenawy, Ayman E. Arch Med Sci Basic Research INTRODUCTION: One of the plants that has long been considered by humans is Equisetum arvense L. Equisetum arvense L is now recommended for external use to heal wounds and for internal use to relieve urinary tract and prostate disorders. In the current study, the antioxidant, cytotoxicity, and anti-human lung cancer properties of Equisetum arvense were investigated in in vitro conditions. MATERIAL AND METHODS: Total phenolic content, total flavonoid content, radical scavenging activity, and ferrous ion chelating were assessed to evaluate the antioxidant activity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was chosen to investigate anticancer activity of the plant extract. RESULTS: The plant extract scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) as a free radical with an IC(50) of 12.3 ±0.7 µg/ml better than positive controls. The plant was also rich in phenolic compounds with an amount of 396.2 ±3.2 mg GAE/g for total phenolic content. In the MTT assay, human colorectal carcinoma (HCT-8 [HRT-18], Ramos.2G6.4C10, HT-29, and HCT 116) and normal cell lines (HUVEC) were used to study the cytotoxicity and anticancer potential of Equisetum arvense L against human colorectal cancer. CONCLUSIONS: The cell viability of Equisetum arvense L was very low against human colorectal carcinoma cell lines without any cytotoxicity towards the normal (HUVEC) cell line. The best anti-human colorectal carcinoma properties of Equisetum arvense L against the above cell lines were observed in the case of the HT 29 cell line. Termedia Publishing House 2021-06-08 /pmc/articles/PMC10507773/ /pubmed/37732051 http://dx.doi.org/10.5114/aoms/138146 Text en Copyright: © 2021 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Basic Research
Wang, Lei
Zhang, Luojun
Zheng, Guangtao
Luo, Haiping
El-kott, Attalla F.
El-kenawy, Ayman E.
Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title_full Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title_fullStr Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title_full_unstemmed Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title_short Equisetum arvense L aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
title_sort equisetum arvense l aqueous extract: a novel chemotherapeutic supplement for treatment of human colon carcinoma
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507773/
https://www.ncbi.nlm.nih.gov/pubmed/37732051
http://dx.doi.org/10.5114/aoms/138146
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