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Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua
Artemisia annua is the only known plant source of the potent antimalarial artemisinin, which occurs as the low- and high-artemisinin producing (LAP and HAP) chemotypes. Nevertheless, the different mechanisms of artemisinin producing between these two chemotypes were still not fully understood. Here,...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508037/ https://www.ncbi.nlm.nih.gov/pubmed/37731862 http://dx.doi.org/10.1093/hr/uhad164 |
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author | Wang, Xingwen Wu, Lan Xiang, Li Gao, Ranran Yin, Qinggang Wang, Mengyue Liu, Zhaoyu Leng, Liang Su, Yanyan Wan, Huihua Ma, Tingyu Chen, Shilin Shi, Yuhua |
author_facet | Wang, Xingwen Wu, Lan Xiang, Li Gao, Ranran Yin, Qinggang Wang, Mengyue Liu, Zhaoyu Leng, Liang Su, Yanyan Wan, Huihua Ma, Tingyu Chen, Shilin Shi, Yuhua |
author_sort | Wang, Xingwen |
collection | PubMed |
description | Artemisia annua is the only known plant source of the potent antimalarial artemisinin, which occurs as the low- and high-artemisinin producing (LAP and HAP) chemotypes. Nevertheless, the different mechanisms of artemisinin producing between these two chemotypes were still not fully understood. Here, we performed a comprehensive analysis of genome resequencing, metabolome, and transcriptome data to systematically compare the difference in the LAP chemotype JL and HAP chemotype HAN. Metabolites analysis revealed that 72.18% of sesquiterpenes was highly accumulated in HAN compared to JL. Integrated omics analysis found a DBR2-Like (DBR2L) gene may be involved in artemisinin biosynthesis. DBR2L was highly homologous with DBR2, belonged to ORR3 family, and had the DBR2 activity of catalyzing artemisinic aldehyde to dihydroartemisinic aldehyde. Genome resequencing and promoter cloning revealed that complicated variations existed in DBR2L promoters among different varieties of A. annua and were clustered into three variation types. The promoter activity of diverse variant types showed obvious differences. Furthermore, the core region (−625 to 0) of the DBR2L promoter was identified and candidate transcription factors involved in DBR2L regulation were screened. Thus, the result indicates that DBR2L is another key enzyme involved in artemisinin biosynthesis. The promoter variation in DBR2L affects its expression level, and thereby may result in the different yield of artemisinin in varieties of A. annua. It provides a novel insight into the mechanism of artemisinin-producing difference in LAP and HAP chemotypes of A. annua, and will assist in a high yield of artemisinin in A. annua. |
format | Online Article Text |
id | pubmed-10508037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105080372023-09-20 Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua Wang, Xingwen Wu, Lan Xiang, Li Gao, Ranran Yin, Qinggang Wang, Mengyue Liu, Zhaoyu Leng, Liang Su, Yanyan Wan, Huihua Ma, Tingyu Chen, Shilin Shi, Yuhua Hortic Res Article Artemisia annua is the only known plant source of the potent antimalarial artemisinin, which occurs as the low- and high-artemisinin producing (LAP and HAP) chemotypes. Nevertheless, the different mechanisms of artemisinin producing between these two chemotypes were still not fully understood. Here, we performed a comprehensive analysis of genome resequencing, metabolome, and transcriptome data to systematically compare the difference in the LAP chemotype JL and HAP chemotype HAN. Metabolites analysis revealed that 72.18% of sesquiterpenes was highly accumulated in HAN compared to JL. Integrated omics analysis found a DBR2-Like (DBR2L) gene may be involved in artemisinin biosynthesis. DBR2L was highly homologous with DBR2, belonged to ORR3 family, and had the DBR2 activity of catalyzing artemisinic aldehyde to dihydroartemisinic aldehyde. Genome resequencing and promoter cloning revealed that complicated variations existed in DBR2L promoters among different varieties of A. annua and were clustered into three variation types. The promoter activity of diverse variant types showed obvious differences. Furthermore, the core region (−625 to 0) of the DBR2L promoter was identified and candidate transcription factors involved in DBR2L regulation were screened. Thus, the result indicates that DBR2L is another key enzyme involved in artemisinin biosynthesis. The promoter variation in DBR2L affects its expression level, and thereby may result in the different yield of artemisinin in varieties of A. annua. It provides a novel insight into the mechanism of artemisinin-producing difference in LAP and HAP chemotypes of A. annua, and will assist in a high yield of artemisinin in A. annua. Oxford University Press 2023-08-16 /pmc/articles/PMC10508037/ /pubmed/37731862 http://dx.doi.org/10.1093/hr/uhad164 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Wang, Xingwen Wu, Lan Xiang, Li Gao, Ranran Yin, Qinggang Wang, Mengyue Liu, Zhaoyu Leng, Liang Su, Yanyan Wan, Huihua Ma, Tingyu Chen, Shilin Shi, Yuhua Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title | Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title_full | Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title_fullStr | Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title_full_unstemmed | Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title_short | Promoter variations in DBR2-like affect artemisinin production in different chemotypes of Artemisia annua |
title_sort | promoter variations in dbr2-like affect artemisinin production in different chemotypes of artemisia annua |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508037/ https://www.ncbi.nlm.nih.gov/pubmed/37731862 http://dx.doi.org/10.1093/hr/uhad164 |
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