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Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation

Every tissue has an extracellular matrix (ECM) with certain properties unique to it – the tissue ‘niche’ – that are necessary for normal function. A distinct specific population of quiescent keratocan-expressing keratocytes populate the corneal stroma during homeostasis to maintain corneal function....

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Autores principales: Acosta, Ana C., Sun, Mei, Zafrullah, Nabeel, Avila, Marcel Y., Margo, Curtis E., Espana, Edgar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508697/
https://www.ncbi.nlm.nih.gov/pubmed/37702214
http://dx.doi.org/10.1242/dmm.050090
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author Acosta, Ana C.
Sun, Mei
Zafrullah, Nabeel
Avila, Marcel Y.
Margo, Curtis E.
Espana, Edgar M.
author_facet Acosta, Ana C.
Sun, Mei
Zafrullah, Nabeel
Avila, Marcel Y.
Margo, Curtis E.
Espana, Edgar M.
author_sort Acosta, Ana C.
collection PubMed
description Every tissue has an extracellular matrix (ECM) with certain properties unique to it – the tissue ‘niche’ – that are necessary for normal function. A distinct specific population of quiescent keratocan-expressing keratocytes populate the corneal stroma during homeostasis to maintain corneal function. However, during wound healing, when there is alteration of the niche conditions, keratocytes undergo apoptosis, and activated corneal fibroblasts and myofibroblasts attempt to restore tissue integrity and function. It is unknown what the fate of activated and temporary fibroblasts and myofibroblasts is after the wound healing process has resolved. In this study, we used several strategies to elucidate the cellular dynamics of corneal wound healing and the fate of corneal fibroblasts. We injured the cornea of a novel mouse model that allows cell-lineage tracing, and we transplanted a cell suspension of in vitro-expanded corneal fibroblasts that could be tracked after being relocated into normal stroma. These transplanted fibroblasts regained expression of keratocan in vivo when relocated to a normal stromal niche. These findings suggest that transformed fibroblasts maintain plasticity and can be induced to a keratocyte phenotype once relocated to an ECM with normal signaling ECM.
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spelling pubmed-105086972023-09-20 Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation Acosta, Ana C. Sun, Mei Zafrullah, Nabeel Avila, Marcel Y. Margo, Curtis E. Espana, Edgar M. Dis Model Mech Research Article Every tissue has an extracellular matrix (ECM) with certain properties unique to it – the tissue ‘niche’ – that are necessary for normal function. A distinct specific population of quiescent keratocan-expressing keratocytes populate the corneal stroma during homeostasis to maintain corneal function. However, during wound healing, when there is alteration of the niche conditions, keratocytes undergo apoptosis, and activated corneal fibroblasts and myofibroblasts attempt to restore tissue integrity and function. It is unknown what the fate of activated and temporary fibroblasts and myofibroblasts is after the wound healing process has resolved. In this study, we used several strategies to elucidate the cellular dynamics of corneal wound healing and the fate of corneal fibroblasts. We injured the cornea of a novel mouse model that allows cell-lineage tracing, and we transplanted a cell suspension of in vitro-expanded corneal fibroblasts that could be tracked after being relocated into normal stroma. These transplanted fibroblasts regained expression of keratocan in vivo when relocated to a normal stromal niche. These findings suggest that transformed fibroblasts maintain plasticity and can be induced to a keratocyte phenotype once relocated to an ECM with normal signaling ECM. The Company of Biologists Ltd 2023-09-13 /pmc/articles/PMC10508697/ /pubmed/37702214 http://dx.doi.org/10.1242/dmm.050090 Text en © 2023. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Acosta, Ana C.
Sun, Mei
Zafrullah, Nabeel
Avila, Marcel Y.
Margo, Curtis E.
Espana, Edgar M.
Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title_full Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title_fullStr Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title_full_unstemmed Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title_short Stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
title_sort stromal matrix directs corneal fibroblasts to re-express keratocan after injury and transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508697/
https://www.ncbi.nlm.nih.gov/pubmed/37702214
http://dx.doi.org/10.1242/dmm.050090
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