A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction...

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Autores principales: Liu, Jian, Qian, Weidong, Wang, Jian, Bai, Yilan, Gui, Yaping, Xia, Luming, Gong, Guohua, Ge, Feifei, Shen, Haixiao, Chang, Xiaojing, Zhao, Hongjin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508970/
https://www.ncbi.nlm.nih.gov/pubmed/37725895
http://dx.doi.org/10.33073/pjm-2023-029
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author Liu, Jian
Qian, Weidong
Wang, Jian
Bai, Yilan
Gui, Yaping
Xia, Luming
Gong, Guohua
Ge, Feifei
Shen, Haixiao
Chang, Xiaojing
Zhao, Hongjin
author_facet Liu, Jian
Qian, Weidong
Wang, Jian
Bai, Yilan
Gui, Yaping
Xia, Luming
Gong, Guohua
Ge, Feifei
Shen, Haixiao
Chang, Xiaojing
Zhao, Hongjin
author_sort Liu, Jian
collection PubMed
description Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.
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spelling pubmed-105089702023-09-20 A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis Liu, Jian Qian, Weidong Wang, Jian Bai, Yilan Gui, Yaping Xia, Luming Gong, Guohua Ge, Feifei Shen, Haixiao Chang, Xiaojing Zhao, Hongjin Pol J Microbiol Short Communication Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis. Sciendo 2023-09-20 /pmc/articles/PMC10508970/ /pubmed/37725895 http://dx.doi.org/10.33073/pjm-2023-029 Text en © 2023 Jian Liu et al., published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Short Communication
Liu, Jian
Qian, Weidong
Wang, Jian
Bai, Yilan
Gui, Yaping
Xia, Luming
Gong, Guohua
Ge, Feifei
Shen, Haixiao
Chang, Xiaojing
Zhao, Hongjin
A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title_full A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title_fullStr A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title_full_unstemmed A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title_short A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis
title_sort recombinase-aided amplification assay for the detection of chlamydia felis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10508970/
https://www.ncbi.nlm.nih.gov/pubmed/37725895
http://dx.doi.org/10.33073/pjm-2023-029
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