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Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region

Individual dispersal plays an important role in preserving genetic diversity in density‐fluctuating populations of arvicoline rodents. When habitats are fragmented and dispersal between habitats is severely constrained, genetic diversity can be lost. Here, I investigated whether genetic diversity in...

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Autor principal: Ishibashi, Yasuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509600/
https://www.ncbi.nlm.nih.gov/pubmed/37736279
http://dx.doi.org/10.1002/ece3.10472
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author Ishibashi, Yasuyuki
author_facet Ishibashi, Yasuyuki
author_sort Ishibashi, Yasuyuki
collection PubMed
description Individual dispersal plays an important role in preserving genetic diversity in density‐fluctuating populations of arvicoline rodents. When habitats are fragmented and dispersal between habitats is severely constrained, genetic diversity can be lost. Here, I investigated whether genetic diversity in the gray‐sided vole Myodes rufocanus was preserved in an intensive farming region in Japan, where voles inhabited isolated windbreak forests along the borders of plowed lands. Genetic structure was examined in 673 vole samples (330 in spring and 343 in fall) collected at 34 windbreak forests located 0.35–20 km apart. A part of the control region (425 bp) of mitochondrial DNA (mtDNA) was sequenced in 673 voles, yielding 76 haplotypes. Genetic differentiation of maternally inherited mtDNA among trapping sites was markedly lower in males than in females in both seasons, indicating strong male‐biased dispersal. Genotypes at six microsatellite DNA loci were determined in 494 voles (245 in spring and 249 in fall) from 18 trapping sites, and loci harbored 16–24 alleles. The mean number of alleles per locus (allelic diversity) at trapping sites was positively correlated with the number of examined individuals (density) in both seasons, and the relationship was very similar to that of a previous study performed in much less fragmented populations. The genetic differentiation of microsatellite DNA among trapping sites decreased considerably from spring to fall. In a STRUCTURE analysis with a most probable cluster number of two, closer trapping sites showed more similar mean values of cluster admixture proportions. The present findings indicate that gene flow among isolated windbreak forests, which occurred mainly by dispersal of males, was not restrained in this intensive farming region. Furthermore, the results suggest that genetic diversity in the study population was preserved as well as in less fragmented populations.
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spelling pubmed-105096002023-09-21 Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region Ishibashi, Yasuyuki Ecol Evol Research Articles Individual dispersal plays an important role in preserving genetic diversity in density‐fluctuating populations of arvicoline rodents. When habitats are fragmented and dispersal between habitats is severely constrained, genetic diversity can be lost. Here, I investigated whether genetic diversity in the gray‐sided vole Myodes rufocanus was preserved in an intensive farming region in Japan, where voles inhabited isolated windbreak forests along the borders of plowed lands. Genetic structure was examined in 673 vole samples (330 in spring and 343 in fall) collected at 34 windbreak forests located 0.35–20 km apart. A part of the control region (425 bp) of mitochondrial DNA (mtDNA) was sequenced in 673 voles, yielding 76 haplotypes. Genetic differentiation of maternally inherited mtDNA among trapping sites was markedly lower in males than in females in both seasons, indicating strong male‐biased dispersal. Genotypes at six microsatellite DNA loci were determined in 494 voles (245 in spring and 249 in fall) from 18 trapping sites, and loci harbored 16–24 alleles. The mean number of alleles per locus (allelic diversity) at trapping sites was positively correlated with the number of examined individuals (density) in both seasons, and the relationship was very similar to that of a previous study performed in much less fragmented populations. The genetic differentiation of microsatellite DNA among trapping sites decreased considerably from spring to fall. In a STRUCTURE analysis with a most probable cluster number of two, closer trapping sites showed more similar mean values of cluster admixture proportions. The present findings indicate that gene flow among isolated windbreak forests, which occurred mainly by dispersal of males, was not restrained in this intensive farming region. Furthermore, the results suggest that genetic diversity in the study population was preserved as well as in less fragmented populations. John Wiley and Sons Inc. 2023-09-19 /pmc/articles/PMC10509600/ /pubmed/37736279 http://dx.doi.org/10.1002/ece3.10472 Text en © 2023 The Author. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Ishibashi, Yasuyuki
Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title_full Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title_fullStr Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title_full_unstemmed Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title_short Preservation of genetic diversity in a highly fragmented population of the gray‐sided vole Myodes rufocanus in an intensive farming region
title_sort preservation of genetic diversity in a highly fragmented population of the gray‐sided vole myodes rufocanus in an intensive farming region
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509600/
https://www.ncbi.nlm.nih.gov/pubmed/37736279
http://dx.doi.org/10.1002/ece3.10472
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