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Complete genome of Aedes japonicus narnavirus from wild caught mosquitoes collected in Ohio, USA.
Narnaviruses infect several genera of mosquitoes including Culex and Aedes . The narnavirus genome is a positive, single stranded RNA encoding an RNA-dependent RNA polymerase gene. The partial genome of a narnavirus identified in wild Aedes japonicus mosquitoes collected in Wooster, Ohio, USA was ob...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509690/ https://www.ncbi.nlm.nih.gov/pubmed/37736247 http://dx.doi.org/10.17912/micropub.biology.000909 |
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author | Nanfack-Minkeu, Ferdinand Delong, Alexander Morgan, William |
author_facet | Nanfack-Minkeu, Ferdinand Delong, Alexander Morgan, William |
author_sort | Nanfack-Minkeu, Ferdinand |
collection | PubMed |
description | Narnaviruses infect several genera of mosquitoes including Culex and Aedes . The narnavirus genome is a positive, single stranded RNA encoding an RNA-dependent RNA polymerase gene. The partial genome of a narnavirus identified in wild Aedes japonicus mosquitoes collected in Wooster, Ohio, USA was obtained using metagenomic analyses. Rapid amplification of 5’-cDNA ends (RACE) and Sanger sequencing were used to obtain the remaining genomic sequence of this strain. The complete genome is composed of 3153 nucleotides and has 98.4% and 99.1% nucleotide sequence identity with Aedes japonicus narnavirus genomes identified in Netherlands and Japan. |
format | Online Article Text |
id | pubmed-10509690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-105096902023-09-21 Complete genome of Aedes japonicus narnavirus from wild caught mosquitoes collected in Ohio, USA. Nanfack-Minkeu, Ferdinand Delong, Alexander Morgan, William MicroPubl Biol Data Updates Narnaviruses infect several genera of mosquitoes including Culex and Aedes . The narnavirus genome is a positive, single stranded RNA encoding an RNA-dependent RNA polymerase gene. The partial genome of a narnavirus identified in wild Aedes japonicus mosquitoes collected in Wooster, Ohio, USA was obtained using metagenomic analyses. Rapid amplification of 5’-cDNA ends (RACE) and Sanger sequencing were used to obtain the remaining genomic sequence of this strain. The complete genome is composed of 3153 nucleotides and has 98.4% and 99.1% nucleotide sequence identity with Aedes japonicus narnavirus genomes identified in Netherlands and Japan. Caltech Library 2023-09-05 /pmc/articles/PMC10509690/ /pubmed/37736247 http://dx.doi.org/10.17912/micropub.biology.000909 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Data Updates Nanfack-Minkeu, Ferdinand Delong, Alexander Morgan, William Complete genome of Aedes japonicus narnavirus from wild caught mosquitoes collected in Ohio, USA. |
title |
Complete genome of
Aedes japonicus
narnavirus from wild caught mosquitoes collected in Ohio, USA.
|
title_full |
Complete genome of
Aedes japonicus
narnavirus from wild caught mosquitoes collected in Ohio, USA.
|
title_fullStr |
Complete genome of
Aedes japonicus
narnavirus from wild caught mosquitoes collected in Ohio, USA.
|
title_full_unstemmed |
Complete genome of
Aedes japonicus
narnavirus from wild caught mosquitoes collected in Ohio, USA.
|
title_short |
Complete genome of
Aedes japonicus
narnavirus from wild caught mosquitoes collected in Ohio, USA.
|
title_sort | complete genome of
aedes japonicus
narnavirus from wild caught mosquitoes collected in ohio, usa. |
topic | Data Updates |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509690/ https://www.ncbi.nlm.nih.gov/pubmed/37736247 http://dx.doi.org/10.17912/micropub.biology.000909 |
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