Generation of mouse models carrying B cell restricted single or multiplexed loss-of-function mutations through CRISPR-Cas9 gene editing

Here, we present a protocol to generate B cell restricted mouse models of loss-of-function genetic drivers typical of lymphoproliferative disorders, using stem cell engineering of murine strains carrying B cell restricted Cas9 expression. We describe steps for preparing lentivirus expressing sgRNA-m...

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Detalles Bibliográficos
Autores principales: ten Hacken, Elisa, Gruber, Michaela, Hernández-Sánchez, María, Hoffmann, Gabriela Brunsting, Baranowski, Kaitlyn, Redd, Robert A., Clement, Kendell, Livak, Kenneth, Wu, Catherine J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510057/
https://www.ncbi.nlm.nih.gov/pubmed/37729058
http://dx.doi.org/10.1016/j.xpro.2023.102165

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