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Protocol to analyze chromatin-bound proteins through the cell cycle using Chromoflow flow cytometry
Chromatin-bound proteins have been conventionally measured through subcellular fractionation followed by immunoblotting or by immunofluorescence microscopy. Here, we present Chromoflow, a protocol for the quantitative analyses of protein levels on chromatin in single cells and throughout the cell cy...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510066/ https://www.ncbi.nlm.nih.gov/pubmed/37725510 http://dx.doi.org/10.1016/j.xpro.2023.102568 |