Cargando…

Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens

BACKGROUND: Eimeria tenella is an obligate intracellular parasitic protozoan that invades the chicken cecum and causes coccidiosis, which induces acute lesions and weight loss. Elucidating the anticoccidial mechanism of action of green tea polyphenols could aid the development of anticoccidial drugs...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Hai-Yang, Deng, Meng-Ling, Yang, Jian-Fa, Ma, Jun, Shu, Fan-Fan, Cheng, Wen-Jie, Zhu, Xing-Quan, Zou, Feng-Cai, He, Jun-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510215/
https://www.ncbi.nlm.nih.gov/pubmed/37726789
http://dx.doi.org/10.1186/s13071-023-05922-x
_version_ 1785107919286566912
author Song, Hai-Yang
Deng, Meng-Ling
Yang, Jian-Fa
Ma, Jun
Shu, Fan-Fan
Cheng, Wen-Jie
Zhu, Xing-Quan
Zou, Feng-Cai
He, Jun-Jun
author_facet Song, Hai-Yang
Deng, Meng-Ling
Yang, Jian-Fa
Ma, Jun
Shu, Fan-Fan
Cheng, Wen-Jie
Zhu, Xing-Quan
Zou, Feng-Cai
He, Jun-Jun
author_sort Song, Hai-Yang
collection PubMed
description BACKGROUND: Eimeria tenella is an obligate intracellular parasitic protozoan that invades the chicken cecum and causes coccidiosis, which induces acute lesions and weight loss. Elucidating the anticoccidial mechanism of action of green tea polyphenols could aid the development of anticoccidial drugs and resolve the problem of drug resistance in E. tenella. METHODS: We constructed a model of E. tenella infection in Wuliangshan black-boned chickens, an indigenous breed of Yunnan Province, China, to study the efficacy of green tea polyphenols against the infection. Alterations in gene expression and in the microbial flora in the cecum were analyzed by ribonucleic acid (RNA) sequencing and 16S ribosomal RNA (rRNA) sequencing. Quantitative real-time polymerase chain reaction was used to verify the host gene expression data obtained by RNA sequencing. Network pharmacology and molecular docking were used to clarify the interactions between the component green tea polyphenols and the targeted proteins; potential anticoccidial herbs were also analyzed. RESULTS: Treatment with the green tea polyphenols led to a reduction in the lesion score and weight loss of the chickens induced by E. tenella infection. The expression of matrix metalloproteinase 7 (MMP7), MMP1, nitric oxide synthase 2 and ephrin type-A receptor 2 was significantly altered in the E. tenella infection plus green tea polyphenol-treated group and in the E. tenella infection group compared with the control group; these genes were also predicted targets of tea polyphenols. Furthermore, the tea polyphenol (-)-epigallocatechin gallate acted on most of the targets, and the molecular docking analysis showed that it has good affinity with interferon induced with helicase C domain 1 protein. 16S ribosomal RNA sequencing showed that the green tea polyphenols had a regulatory effect on changes in the fecal microbiota induced by E. tenella infection. In total, 171 herbs were predicted to act on two or three targets in MMP7, MMP1, nitric oxide synthase 2 and ephrin type-A receptor 2. CONCLUSIONS: Green tea polyphenols can directly or indirectly regulate host gene expression and alter the growth of microbiota. The results presented here shed light on the mechanism of action of green tea polyphenols against E. tenella infection in chickens, and have implications for the development of novel anticoccidial products. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05922-x.
format Online
Article
Text
id pubmed-10510215
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-105102152023-09-21 Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens Song, Hai-Yang Deng, Meng-Ling Yang, Jian-Fa Ma, Jun Shu, Fan-Fan Cheng, Wen-Jie Zhu, Xing-Quan Zou, Feng-Cai He, Jun-Jun Parasit Vectors Research BACKGROUND: Eimeria tenella is an obligate intracellular parasitic protozoan that invades the chicken cecum and causes coccidiosis, which induces acute lesions and weight loss. Elucidating the anticoccidial mechanism of action of green tea polyphenols could aid the development of anticoccidial drugs and resolve the problem of drug resistance in E. tenella. METHODS: We constructed a model of E. tenella infection in Wuliangshan black-boned chickens, an indigenous breed of Yunnan Province, China, to study the efficacy of green tea polyphenols against the infection. Alterations in gene expression and in the microbial flora in the cecum were analyzed by ribonucleic acid (RNA) sequencing and 16S ribosomal RNA (rRNA) sequencing. Quantitative real-time polymerase chain reaction was used to verify the host gene expression data obtained by RNA sequencing. Network pharmacology and molecular docking were used to clarify the interactions between the component green tea polyphenols and the targeted proteins; potential anticoccidial herbs were also analyzed. RESULTS: Treatment with the green tea polyphenols led to a reduction in the lesion score and weight loss of the chickens induced by E. tenella infection. The expression of matrix metalloproteinase 7 (MMP7), MMP1, nitric oxide synthase 2 and ephrin type-A receptor 2 was significantly altered in the E. tenella infection plus green tea polyphenol-treated group and in the E. tenella infection group compared with the control group; these genes were also predicted targets of tea polyphenols. Furthermore, the tea polyphenol (-)-epigallocatechin gallate acted on most of the targets, and the molecular docking analysis showed that it has good affinity with interferon induced with helicase C domain 1 protein. 16S ribosomal RNA sequencing showed that the green tea polyphenols had a regulatory effect on changes in the fecal microbiota induced by E. tenella infection. In total, 171 herbs were predicted to act on two or three targets in MMP7, MMP1, nitric oxide synthase 2 and ephrin type-A receptor 2. CONCLUSIONS: Green tea polyphenols can directly or indirectly regulate host gene expression and alter the growth of microbiota. The results presented here shed light on the mechanism of action of green tea polyphenols against E. tenella infection in chickens, and have implications for the development of novel anticoccidial products. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05922-x. BioMed Central 2023-09-19 /pmc/articles/PMC10510215/ /pubmed/37726789 http://dx.doi.org/10.1186/s13071-023-05922-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Song, Hai-Yang
Deng, Meng-Ling
Yang, Jian-Fa
Ma, Jun
Shu, Fan-Fan
Cheng, Wen-Jie
Zhu, Xing-Quan
Zou, Feng-Cai
He, Jun-Jun
Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title_full Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title_fullStr Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title_full_unstemmed Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title_short Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens
title_sort transcriptomic, 16s ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against eimeria tenella infection in wuliangshan black-boned chickens
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510215/
https://www.ncbi.nlm.nih.gov/pubmed/37726789
http://dx.doi.org/10.1186/s13071-023-05922-x
work_keys_str_mv AT songhaiyang transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT dengmengling transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT yangjianfa transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT majun transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT shufanfan transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT chengwenjie transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT zhuxingquan transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT zoufengcai transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens
AT hejunjun transcriptomic16sribosomalribonucleicacidandnetworkpharmacologyanalysesshedlightontheanticoccidialmechanismofgreenteapolyphenolsagainsteimeriatenellainfectioninwuliangshanblackbonedchickens