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Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors
Cytomegalovirus (CMV) mutations associated with antiviral resistance have become a major problem related to high mortality in kidney transplant patients. The aim of the study was to investigate mutations in the CMV genes UL97 and UL54 associated with antiviral resistance. A retrospective observation...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510340/ https://www.ncbi.nlm.nih.gov/pubmed/37724822 http://dx.doi.org/10.1177/09636897231195245 |
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author | Sant’ Anna, Carla de Castro Migone, Silvia Regina da Cruz da Rocha, Carlos Alberto Machado Mello Júnior, Fernando Augusto Rodrigues Seabra, Aline Damasceno Pontes, Thaís Brilhante Rodrigues, João Marildo Soares, Salomé Aparecida Rego, Viviane de Paiva Figueira, Joana Paula Rodrigues, Ana Paula Monteiro Burbano, Rommel Mario Rodriguez |
author_facet | Sant’ Anna, Carla de Castro Migone, Silvia Regina da Cruz da Rocha, Carlos Alberto Machado Mello Júnior, Fernando Augusto Rodrigues Seabra, Aline Damasceno Pontes, Thaís Brilhante Rodrigues, João Marildo Soares, Salomé Aparecida Rego, Viviane de Paiva Figueira, Joana Paula Rodrigues, Ana Paula Monteiro Burbano, Rommel Mario Rodriguez |
author_sort | Sant’ Anna, Carla de Castro |
collection | PubMed |
description | Cytomegalovirus (CMV) mutations associated with antiviral resistance have become a major problem related to high mortality in kidney transplant patients. The aim of the study was to investigate mutations in the CMV genes UL97 and UL54 associated with antiviral resistance. A retrospective observational cohort study was carried out at Hospital Ophir Loyola (HOL), a reference in Kidney Transplantation. A total of 81 patients who underwent kidney transplantation were followed up between 2016 and 2018 were monitored for CMV viral load by performing qPCR. Sanger sequencing was performed on 66 patients. All CMV-positive kidney transplant recipients were included. Mutations were observed in 15 samples (22.72%) from patients. Most cases involved UL97 mutations. Mutation in UL54 without mutation in UL97 was detected in only 2 cases. Resistance mutations in UL97 were identified, such as M460V, L595S, H520Q, two co-mutations D465R + Del524 and A594P + D413A and a 3 codon deletion (del598-601). The search for mutations in the CMV genes identified mutations that confer resistance to conventional antivirals, such as ganciclovir and cidofovir, used in the treatment of these patients. Confirmation of the association with increased CMV viral load in transplanted patients, due to mutation in resistance genes, requires phenotypic analysis for confirmation purposes. These were the first findings in patients in northern Brazil that we know of. |
format | Online Article Text |
id | pubmed-10510340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-105103402023-09-21 Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors Sant’ Anna, Carla de Castro Migone, Silvia Regina da Cruz da Rocha, Carlos Alberto Machado Mello Júnior, Fernando Augusto Rodrigues Seabra, Aline Damasceno Pontes, Thaís Brilhante Rodrigues, João Marildo Soares, Salomé Aparecida Rego, Viviane de Paiva Figueira, Joana Paula Rodrigues, Ana Paula Monteiro Burbano, Rommel Mario Rodriguez Cell Transplant Letter to the Editor Cytomegalovirus (CMV) mutations associated with antiviral resistance have become a major problem related to high mortality in kidney transplant patients. The aim of the study was to investigate mutations in the CMV genes UL97 and UL54 associated with antiviral resistance. A retrospective observational cohort study was carried out at Hospital Ophir Loyola (HOL), a reference in Kidney Transplantation. A total of 81 patients who underwent kidney transplantation were followed up between 2016 and 2018 were monitored for CMV viral load by performing qPCR. Sanger sequencing was performed on 66 patients. All CMV-positive kidney transplant recipients were included. Mutations were observed in 15 samples (22.72%) from patients. Most cases involved UL97 mutations. Mutation in UL54 without mutation in UL97 was detected in only 2 cases. Resistance mutations in UL97 were identified, such as M460V, L595S, H520Q, two co-mutations D465R + Del524 and A594P + D413A and a 3 codon deletion (del598-601). The search for mutations in the CMV genes identified mutations that confer resistance to conventional antivirals, such as ganciclovir and cidofovir, used in the treatment of these patients. Confirmation of the association with increased CMV viral load in transplanted patients, due to mutation in resistance genes, requires phenotypic analysis for confirmation purposes. These were the first findings in patients in northern Brazil that we know of. SAGE Publications 2023-09-19 /pmc/articles/PMC10510340/ /pubmed/37724822 http://dx.doi.org/10.1177/09636897231195245 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Letter to the Editor Sant’ Anna, Carla de Castro Migone, Silvia Regina da Cruz da Rocha, Carlos Alberto Machado Mello Júnior, Fernando Augusto Rodrigues Seabra, Aline Damasceno Pontes, Thaís Brilhante Rodrigues, João Marildo Soares, Salomé Aparecida Rego, Viviane de Paiva Figueira, Joana Paula Rodrigues, Ana Paula Monteiro Burbano, Rommel Mario Rodriguez Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title | Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title_full | Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title_fullStr | Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title_full_unstemmed | Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title_short | Research for Cytomegalovirus Mutations Associated With Resistance to Antivirals in Kidney Transplant Receptors |
title_sort | research for cytomegalovirus mutations associated with resistance to antivirals in kidney transplant receptors |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510340/ https://www.ncbi.nlm.nih.gov/pubmed/37724822 http://dx.doi.org/10.1177/09636897231195245 |
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