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Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential

In this study holmium oxide nanoparticles (Ho(2)O(3) NPs) are fabricated using Hyphaene thebaica extracts as a bioreductant. The XRD pattern of HT-Ho(2)O(3) NPs (product from phyto-reduction) suggested that the nanoparticles are crystalline with no impurities. Scherrer approximation revealed grain s...

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Autores principales: Ahmed Mohamed, Hamze Elsayed, Khalil, Ali Talha, Hkiri, Khaoula, Ayaz, Muhammad, Usman, Assad, Sadiq, Abdul, Ullah, Farhat, Hussain, Ishtiaq, Maaza, Malik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510458/
https://www.ncbi.nlm.nih.gov/pubmed/37736569
http://dx.doi.org/10.1039/d3ra05047e
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author Ahmed Mohamed, Hamze Elsayed
Khalil, Ali Talha
Hkiri, Khaoula
Ayaz, Muhammad
Usman, Assad
Sadiq, Abdul
Ullah, Farhat
Hussain, Ishtiaq
Maaza, Malik
author_facet Ahmed Mohamed, Hamze Elsayed
Khalil, Ali Talha
Hkiri, Khaoula
Ayaz, Muhammad
Usman, Assad
Sadiq, Abdul
Ullah, Farhat
Hussain, Ishtiaq
Maaza, Malik
author_sort Ahmed Mohamed, Hamze Elsayed
collection PubMed
description In this study holmium oxide nanoparticles (Ho(2)O(3) NPs) are fabricated using Hyphaene thebaica extracts as a bioreductant. The XRD pattern of HT-Ho(2)O(3) NPs (product from phyto-reduction) suggested that the nanoparticles are crystalline with no impurities. Scherrer approximation revealed grain sizes of ∼10 nm. The HR-TEM revealed HT-Ho(2)O(3) NPs possessed a quasi-spherical morphology complemented by SEM and the particle sizes were in the range of 6–12 nm. The infrared spectra revealed characteristic Ho–O bonding at ∼603 cm(−1). Raman spectra indicated five main peaks positioned at 156 cm(−1), 214 cm(−1), 328 cm(−1), 379 cm(−1) and 607 cm(−1). E(g) (optical bandgap) was found to be 5.1 eV. PL spectra indicated two major peaks at 415 nm and 607 nm. EDS spectra confirmed the elemental presence of holmium (Ho). Spotty rings were obtained during the SAED measurement which indicated crystallinity of HT-Ho(2)O(3) NPs. The HT-Ho(2)O(3) NPs were further analyzed for their antioxidant, anti-angiogenic and cytotoxic properties. The antioxidant potential was moderate i.e., 43.40 ± 0.96% at 1000 μg mL(−1) which decreased in a dose dependent manner. Brine shrimp lethality was highest at 1000 μg mL(−1) with the LC(50) 320.4 μg mL(−1). Moderate anti-angiogenic potential was observed using in ova CAM assay. MTT bioassay revealed that the HT-Ho(2)O(3) NPs inhibited the 3T3 cells (IC(50) 67.9 μg mL(−1)), however, no significant inhibition was observed against MCF-7 cells. α-Amylase and β-glucosidase inhibition revealed that the HT-Ho(2)O(3) NPs can be of use in controlling blood glucose levels. Overall, it can be concluded that biosynthesis using aqueous extracts can be a suitable alternative in finding ecofriendly paradigms for the synthesis of nanoparticles. We suggest extended research into the bioreduced Ho(2)O(3) NPs for establishing their biomedical potential and toxicity.
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spelling pubmed-105104582023-09-21 Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential Ahmed Mohamed, Hamze Elsayed Khalil, Ali Talha Hkiri, Khaoula Ayaz, Muhammad Usman, Assad Sadiq, Abdul Ullah, Farhat Hussain, Ishtiaq Maaza, Malik RSC Adv Chemistry In this study holmium oxide nanoparticles (Ho(2)O(3) NPs) are fabricated using Hyphaene thebaica extracts as a bioreductant. The XRD pattern of HT-Ho(2)O(3) NPs (product from phyto-reduction) suggested that the nanoparticles are crystalline with no impurities. Scherrer approximation revealed grain sizes of ∼10 nm. The HR-TEM revealed HT-Ho(2)O(3) NPs possessed a quasi-spherical morphology complemented by SEM and the particle sizes were in the range of 6–12 nm. The infrared spectra revealed characteristic Ho–O bonding at ∼603 cm(−1). Raman spectra indicated five main peaks positioned at 156 cm(−1), 214 cm(−1), 328 cm(−1), 379 cm(−1) and 607 cm(−1). E(g) (optical bandgap) was found to be 5.1 eV. PL spectra indicated two major peaks at 415 nm and 607 nm. EDS spectra confirmed the elemental presence of holmium (Ho). Spotty rings were obtained during the SAED measurement which indicated crystallinity of HT-Ho(2)O(3) NPs. The HT-Ho(2)O(3) NPs were further analyzed for their antioxidant, anti-angiogenic and cytotoxic properties. The antioxidant potential was moderate i.e., 43.40 ± 0.96% at 1000 μg mL(−1) which decreased in a dose dependent manner. Brine shrimp lethality was highest at 1000 μg mL(−1) with the LC(50) 320.4 μg mL(−1). Moderate anti-angiogenic potential was observed using in ova CAM assay. MTT bioassay revealed that the HT-Ho(2)O(3) NPs inhibited the 3T3 cells (IC(50) 67.9 μg mL(−1)), however, no significant inhibition was observed against MCF-7 cells. α-Amylase and β-glucosidase inhibition revealed that the HT-Ho(2)O(3) NPs can be of use in controlling blood glucose levels. Overall, it can be concluded that biosynthesis using aqueous extracts can be a suitable alternative in finding ecofriendly paradigms for the synthesis of nanoparticles. We suggest extended research into the bioreduced Ho(2)O(3) NPs for establishing their biomedical potential and toxicity. The Royal Society of Chemistry 2023-09-20 /pmc/articles/PMC10510458/ /pubmed/37736569 http://dx.doi.org/10.1039/d3ra05047e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ahmed Mohamed, Hamze Elsayed
Khalil, Ali Talha
Hkiri, Khaoula
Ayaz, Muhammad
Usman, Assad
Sadiq, Abdul
Ullah, Farhat
Hussain, Ishtiaq
Maaza, Malik
Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title_full Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title_fullStr Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title_full_unstemmed Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title_short Phyto-fabrication of ultrafine nanoscale holmium oxide HT-Ho(2)O(3) NPs and their biomedical potential
title_sort phyto-fabrication of ultrafine nanoscale holmium oxide ht-ho(2)o(3) nps and their biomedical potential
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510458/
https://www.ncbi.nlm.nih.gov/pubmed/37736569
http://dx.doi.org/10.1039/d3ra05047e
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