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Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats
OBJECTIVE(S): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques. MATERIALS AND METHODS: Seventy two Sprague-Dawley female...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510480/ https://www.ncbi.nlm.nih.gov/pubmed/37736515 http://dx.doi.org/10.22038/IJBMS.2023.69984.15223 |
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author | Toktay, Erdem Selli, Jale Gurbuz, Muhammet Ali Alaca, Raziye |
author_facet | Toktay, Erdem Selli, Jale Gurbuz, Muhammet Ali Alaca, Raziye |
author_sort | Toktay, Erdem |
collection | PubMed |
description | OBJECTIVE(S): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques. MATERIALS AND METHODS: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically. RESULTS: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-α NF-κB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups. CONCLUSION: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damage |
format | Online Article Text |
id | pubmed-10510480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-105104802023-09-21 Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats Toktay, Erdem Selli, Jale Gurbuz, Muhammet Ali Alaca, Raziye Iran J Basic Med Sci Original Article OBJECTIVE(S): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques. MATERIALS AND METHODS: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically. RESULTS: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-α NF-κB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups. CONCLUSION: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damage Mashhad University of Medical Sciences 2023 /pmc/articles/PMC10510480/ /pubmed/37736515 http://dx.doi.org/10.22038/IJBMS.2023.69984.15223 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Toktay, Erdem Selli, Jale Gurbuz, Muhammet Ali Alaca, Raziye Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title_full | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title_fullStr | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title_full_unstemmed | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title_short | Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats |
title_sort | investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (pcos) in rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510480/ https://www.ncbi.nlm.nih.gov/pubmed/37736515 http://dx.doi.org/10.22038/IJBMS.2023.69984.15223 |
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