Cargando…
Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line
OBJECTIVE(S): Targeting the lytic cycle of the Epstein-Barr virus (EBV) has been considered a new treatment strategy for malignancies caused by this virus. This study aimed to investigate the effect of Dendrosomal NanoCurcumin (DNC) to prevent cell transformation and inhibit the expression of viral...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510484/ https://www.ncbi.nlm.nih.gov/pubmed/37736518 http://dx.doi.org/10.22038/IJBMS.2023.69839.15199 |
_version_ | 1785107977995288576 |
---|---|
author | Cheragh, Mahboobeh Sadeghizadeh, Majid Pouriayevali, Mohammad Hassan Parsania, Masoud |
author_facet | Cheragh, Mahboobeh Sadeghizadeh, Majid Pouriayevali, Mohammad Hassan Parsania, Masoud |
author_sort | Cheragh, Mahboobeh |
collection | PubMed |
description | OBJECTIVE(S): Targeting the lytic cycle of the Epstein-Barr virus (EBV) has been considered a new treatment strategy for malignancies caused by this virus. This study aimed to investigate the effect of Dendrosomal NanoCurcumin (DNC) to prevent cell transformation and inhibit the expression of viral lytic gene expression in the generation of lymphoblastoid cell line (LCL). MATERIALS AND METHODS: Cell viability of LCLs and PBMCs was performed by MTT assay, and flow cytometry (Annexin/PI) was used for evaluation of apoptosis. CD markers on the surface of generated LCL (CD19) cells were examined for cell validation. The effect of DNC on transformation was evaluated by examining cell morphology and determining the expression level of lytic genes BZLF1, Zta, BHRF1, and BRLF1 of EBV using Real-time PCR. Student’s t-test was used for statistical analysis. RESULTS: The MTT assay showed that DNC can inhibit the proliferation of LCL in a dose-dependent manner. The 50% cytotoxic concentration (CC(50)) of DNC and curcumin for LCL was determined 38.8 µg/ml and 75 µg/ml, respectively after 72 hr. Also, Real-time PCR data analysis showed that DNC in 30 µg/ml concentration significantly inhibited cell transformation in the LCL and significantly reduced viral lytic genes such as BZLF1, Zta, BHRF1, and BRLF1expression compared to control. CONCLUSION: Overall, these findings show that DNC reduces the expression of the viral lytic cycle genes and also the induction of cell apoptosis and finally prevents the generation of LCL. |
format | Online Article Text |
id | pubmed-10510484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-105104842023-09-21 Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line Cheragh, Mahboobeh Sadeghizadeh, Majid Pouriayevali, Mohammad Hassan Parsania, Masoud Iran J Basic Med Sci Original Article OBJECTIVE(S): Targeting the lytic cycle of the Epstein-Barr virus (EBV) has been considered a new treatment strategy for malignancies caused by this virus. This study aimed to investigate the effect of Dendrosomal NanoCurcumin (DNC) to prevent cell transformation and inhibit the expression of viral lytic gene expression in the generation of lymphoblastoid cell line (LCL). MATERIALS AND METHODS: Cell viability of LCLs and PBMCs was performed by MTT assay, and flow cytometry (Annexin/PI) was used for evaluation of apoptosis. CD markers on the surface of generated LCL (CD19) cells were examined for cell validation. The effect of DNC on transformation was evaluated by examining cell morphology and determining the expression level of lytic genes BZLF1, Zta, BHRF1, and BRLF1 of EBV using Real-time PCR. Student’s t-test was used for statistical analysis. RESULTS: The MTT assay showed that DNC can inhibit the proliferation of LCL in a dose-dependent manner. The 50% cytotoxic concentration (CC(50)) of DNC and curcumin for LCL was determined 38.8 µg/ml and 75 µg/ml, respectively after 72 hr. Also, Real-time PCR data analysis showed that DNC in 30 µg/ml concentration significantly inhibited cell transformation in the LCL and significantly reduced viral lytic genes such as BZLF1, Zta, BHRF1, and BRLF1expression compared to control. CONCLUSION: Overall, these findings show that DNC reduces the expression of the viral lytic cycle genes and also the induction of cell apoptosis and finally prevents the generation of LCL. Mashhad University of Medical Sciences 2023 /pmc/articles/PMC10510484/ /pubmed/37736518 http://dx.doi.org/10.22038/IJBMS.2023.69839.15199 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cheragh, Mahboobeh Sadeghizadeh, Majid Pouriayevali, Mohammad Hassan Parsania, Masoud Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title | Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title_full | Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title_fullStr | Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title_full_unstemmed | Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title_short | Dendrosomal nanocurcumin prevents EBV-associated cell transformation by targeting the lytic cycle genes of the Epstein-Barr virus in the generation of lymphoblastoid cell line |
title_sort | dendrosomal nanocurcumin prevents ebv-associated cell transformation by targeting the lytic cycle genes of the epstein-barr virus in the generation of lymphoblastoid cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510484/ https://www.ncbi.nlm.nih.gov/pubmed/37736518 http://dx.doi.org/10.22038/IJBMS.2023.69839.15199 |
work_keys_str_mv | AT cheraghmahboobeh dendrosomalnanocurcuminpreventsebvassociatedcelltransformationbytargetingthelyticcyclegenesoftheepsteinbarrvirusinthegenerationoflymphoblastoidcellline AT sadeghizadehmajid dendrosomalnanocurcuminpreventsebvassociatedcelltransformationbytargetingthelyticcyclegenesoftheepsteinbarrvirusinthegenerationoflymphoblastoidcellline AT pouriayevalimohammadhassan dendrosomalnanocurcuminpreventsebvassociatedcelltransformationbytargetingthelyticcyclegenesoftheepsteinbarrvirusinthegenerationoflymphoblastoidcellline AT parsaniamasoud dendrosomalnanocurcuminpreventsebvassociatedcelltransformationbytargetingthelyticcyclegenesoftheepsteinbarrvirusinthegenerationoflymphoblastoidcellline |