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Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats

OBJECTIVE(S): Neuropathy, retinopathy, and nephropathy, known as the triopathy of diabetes, are the consequences of microvascular complications of diabetes. The present study aimed to investigate the potential protective effects of oleanolic acid (OA) administration against diabetic nephropathy cons...

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Autores principales: Iskender, Hatice, Dokumacioglu, Eda, Hayirli, Armagan, Kapakin, Kubra Asena Terim, Bolat, Ismail, Kirman, Esra Manavoglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510490/
https://www.ncbi.nlm.nih.gov/pubmed/37736511
http://dx.doi.org/10.22038/IJBMS.2023.71321.15504
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author Iskender, Hatice
Dokumacioglu, Eda
Hayirli, Armagan
Kapakin, Kubra Asena Terim
Bolat, Ismail
Kirman, Esra Manavoglu
author_facet Iskender, Hatice
Dokumacioglu, Eda
Hayirli, Armagan
Kapakin, Kubra Asena Terim
Bolat, Ismail
Kirman, Esra Manavoglu
author_sort Iskender, Hatice
collection PubMed
description OBJECTIVE(S): Neuropathy, retinopathy, and nephropathy, known as the triopathy of diabetes, are the consequences of microvascular complications of diabetes. The present study aimed to investigate the potential protective effects of oleanolic acid (OA) administration against diabetic nephropathy considering biochemical and histopathological parameters. MATERIALS AND METHODS: The rats with fasting blood glucose levels of 200 mg/dl and above were considered diabetic after induction of diabetes via injecting STZ. The other half of the rats were not injected with STZ (healthy rats). Both healthy and diabetic rats were then divided randomly into two subgroups to be administered with either OA (5 mg/kg) with 1 ml tap water by oral gavage or 1 ml tap water in the same route for 21 days. Serum urea-N, Ca, P, and Mg as well as renal tissue MDA, SOD, NF-κB, IL-6, IL-18, AMPK, YKL-40, and KIM-1 levels were measured. RESULTS: OA administration partially decreased levels of serum urea-N and P, as well as levels of renal tissue MDA and inflammation markers (NF-κB, IL-6, IL-18, YKL-40, and KIM-1) in the diabetic rats. It also partially increased serum Ca and renal tissue AMPK levels in diabetic rats. These positive effects were also seen in renal tissue histopathology. CONCLUSION: OA treatment partially alleviated renal damage inflammatory and oxidative profiles in diabetic rats.
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spelling pubmed-105104902023-09-21 Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats Iskender, Hatice Dokumacioglu, Eda Hayirli, Armagan Kapakin, Kubra Asena Terim Bolat, Ismail Kirman, Esra Manavoglu Iran J Basic Med Sci Original Article OBJECTIVE(S): Neuropathy, retinopathy, and nephropathy, known as the triopathy of diabetes, are the consequences of microvascular complications of diabetes. The present study aimed to investigate the potential protective effects of oleanolic acid (OA) administration against diabetic nephropathy considering biochemical and histopathological parameters. MATERIALS AND METHODS: The rats with fasting blood glucose levels of 200 mg/dl and above were considered diabetic after induction of diabetes via injecting STZ. The other half of the rats were not injected with STZ (healthy rats). Both healthy and diabetic rats were then divided randomly into two subgroups to be administered with either OA (5 mg/kg) with 1 ml tap water by oral gavage or 1 ml tap water in the same route for 21 days. Serum urea-N, Ca, P, and Mg as well as renal tissue MDA, SOD, NF-κB, IL-6, IL-18, AMPK, YKL-40, and KIM-1 levels were measured. RESULTS: OA administration partially decreased levels of serum urea-N and P, as well as levels of renal tissue MDA and inflammation markers (NF-κB, IL-6, IL-18, YKL-40, and KIM-1) in the diabetic rats. It also partially increased serum Ca and renal tissue AMPK levels in diabetic rats. These positive effects were also seen in renal tissue histopathology. CONCLUSION: OA treatment partially alleviated renal damage inflammatory and oxidative profiles in diabetic rats. Mashhad University of Medical Sciences 2023 /pmc/articles/PMC10510490/ /pubmed/37736511 http://dx.doi.org/10.22038/IJBMS.2023.71321.15504 Text en https://creativecommons.org/licenses/by/3.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/ (https://creativecommons.org/licenses/by/3.0/) ) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Iskender, Hatice
Dokumacioglu, Eda
Hayirli, Armagan
Kapakin, Kubra Asena Terim
Bolat, Ismail
Kirman, Esra Manavoglu
Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title_full Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title_fullStr Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title_full_unstemmed Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title_short Effects of oleanolic acid administration on renal NF-kB, IL-18, IL-6, YKL-40, and KIM-1 in experimental diabetic rats
title_sort effects of oleanolic acid administration on renal nf-kb, il-18, il-6, ykl-40, and kim-1 in experimental diabetic rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510490/
https://www.ncbi.nlm.nih.gov/pubmed/37736511
http://dx.doi.org/10.22038/IJBMS.2023.71321.15504
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