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Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications
[Image: see text] For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. My...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510702/ https://www.ncbi.nlm.nih.gov/pubmed/37639711 http://dx.doi.org/10.1021/acsnano.3c05137 |
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author | Salhotra, Aseem Rahman, Mohammad A Ruijgrok, Paul V Meinecke, Christoph R Ušaj, Marko Zemsky, Sasha Lindberg, Frida W Surendiran, Pradheebha Lyttleton, Roman W. Linke, Heiner Korten, Till Bryant, Zev Månsson, Alf |
author_facet | Salhotra, Aseem Rahman, Mohammad A Ruijgrok, Paul V Meinecke, Christoph R Ušaj, Marko Zemsky, Sasha Lindberg, Frida W Surendiran, Pradheebha Lyttleton, Roman W. Linke, Heiner Korten, Till Bryant, Zev Månsson, Alf |
author_sort | Salhotra, Aseem |
collection | PubMed |
description | [Image: see text] For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties. |
format | Online Article Text |
id | pubmed-10510702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105107022023-09-21 Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications Salhotra, Aseem Rahman, Mohammad A Ruijgrok, Paul V Meinecke, Christoph R Ušaj, Marko Zemsky, Sasha Lindberg, Frida W Surendiran, Pradheebha Lyttleton, Roman W. Linke, Heiner Korten, Till Bryant, Zev Månsson, Alf ACS Nano [Image: see text] For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties. American Chemical Society 2023-08-28 /pmc/articles/PMC10510702/ /pubmed/37639711 http://dx.doi.org/10.1021/acsnano.3c05137 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Salhotra, Aseem Rahman, Mohammad A Ruijgrok, Paul V Meinecke, Christoph R Ušaj, Marko Zemsky, Sasha Lindberg, Frida W Surendiran, Pradheebha Lyttleton, Roman W. Linke, Heiner Korten, Till Bryant, Zev Månsson, Alf Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications |
title | Exploitation of
Engineered Light-Switchable Myosin
XI for Nanotechnological Applications |
title_full | Exploitation of
Engineered Light-Switchable Myosin
XI for Nanotechnological Applications |
title_fullStr | Exploitation of
Engineered Light-Switchable Myosin
XI for Nanotechnological Applications |
title_full_unstemmed | Exploitation of
Engineered Light-Switchable Myosin
XI for Nanotechnological Applications |
title_short | Exploitation of
Engineered Light-Switchable Myosin
XI for Nanotechnological Applications |
title_sort | exploitation of
engineered light-switchable myosin
xi for nanotechnological applications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510702/ https://www.ncbi.nlm.nih.gov/pubmed/37639711 http://dx.doi.org/10.1021/acsnano.3c05137 |
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