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Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology
[Image: see text] Streptococcussuis is an important zoonotic pathogen that causes severe invasive disease in pigs and humans. Current methods for genome engineering of S. suis rely on the insertion of antibiotic resistance markers, which is time-consuming and labor-intensive and does not allow the p...
Autores principales: | Gussak, Alex, Ferrando, Maria Laura, Schrama, Mels, van Baarlen, Peter, Wells, Jerry Mark |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10510748/ https://www.ncbi.nlm.nih.gov/pubmed/37602730 http://dx.doi.org/10.1021/acssynbio.3c00110 |
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