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Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device
We developed an innovative millifluidic organ-on-a-chip device, named MINERVA 2.0, that is optically accessible and suitable to serial connection. In the present work, we evaluated MINERVA 2.0 as millifluidic gut epithelium-on-a-chip by using computational modeling and biological assessment. We also...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511260/ https://www.ncbi.nlm.nih.gov/pubmed/37736017 http://dx.doi.org/10.1063/5.0144862 |
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author | Donnaloja, Francesca Izzo, Luca Campanile, Marzia Perottoni, Simone Boeri, Lucia Fanizza, Francesca Sardelli, Lorenzo Jacchetti, Emanuela Raimondi, Manuela T. Rito, Laura Di Craparotta, Ilaria Bolis, Marco Giordano, Carmen Albani, Diego |
author_facet | Donnaloja, Francesca Izzo, Luca Campanile, Marzia Perottoni, Simone Boeri, Lucia Fanizza, Francesca Sardelli, Lorenzo Jacchetti, Emanuela Raimondi, Manuela T. Rito, Laura Di Craparotta, Ilaria Bolis, Marco Giordano, Carmen Albani, Diego |
author_sort | Donnaloja, Francesca |
collection | PubMed |
description | We developed an innovative millifluidic organ-on-a-chip device, named MINERVA 2.0, that is optically accessible and suitable to serial connection. In the present work, we evaluated MINERVA 2.0 as millifluidic gut epithelium-on-a-chip by using computational modeling and biological assessment. We also tested MINERVA 2.0 in a serially connected configuration prodromal to address the complexity of multiorgan interaction. Once cultured under perfusion in our device, human gut immortalized Caco-2 epithelial cells were able to survive at least up to 7 days and form a three-dimensional layer with detectable tight junctions (occludin and zonulin-1 positive). Functional layer development was supported by measurable trans-epithelial resistance and FITC-dextran permeability regulation, together with mucin-2 expression. The dynamic culturing led to a specific transcriptomic profile, assessed by RNASeq, with a total of 524 dysregulated transcripts (191 upregulated and 333 downregulated) between static and dynamic condition. Overall, the collected results suggest that our gut-on-a-chip millifluidic model displays key gut epithelium features and, thanks to its modular design, may be the basis to build a customizable multiorgan-on-a-chip platform. |
format | Online Article Text |
id | pubmed-10511260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-105112602023-09-21 Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device Donnaloja, Francesca Izzo, Luca Campanile, Marzia Perottoni, Simone Boeri, Lucia Fanizza, Francesca Sardelli, Lorenzo Jacchetti, Emanuela Raimondi, Manuela T. Rito, Laura Di Craparotta, Ilaria Bolis, Marco Giordano, Carmen Albani, Diego APL Bioeng Articles We developed an innovative millifluidic organ-on-a-chip device, named MINERVA 2.0, that is optically accessible and suitable to serial connection. In the present work, we evaluated MINERVA 2.0 as millifluidic gut epithelium-on-a-chip by using computational modeling and biological assessment. We also tested MINERVA 2.0 in a serially connected configuration prodromal to address the complexity of multiorgan interaction. Once cultured under perfusion in our device, human gut immortalized Caco-2 epithelial cells were able to survive at least up to 7 days and form a three-dimensional layer with detectable tight junctions (occludin and zonulin-1 positive). Functional layer development was supported by measurable trans-epithelial resistance and FITC-dextran permeability regulation, together with mucin-2 expression. The dynamic culturing led to a specific transcriptomic profile, assessed by RNASeq, with a total of 524 dysregulated transcripts (191 upregulated and 333 downregulated) between static and dynamic condition. Overall, the collected results suggest that our gut-on-a-chip millifluidic model displays key gut epithelium features and, thanks to its modular design, may be the basis to build a customizable multiorgan-on-a-chip platform. AIP Publishing LLC 2023-09-19 /pmc/articles/PMC10511260/ /pubmed/37736017 http://dx.doi.org/10.1063/5.0144862 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Donnaloja, Francesca Izzo, Luca Campanile, Marzia Perottoni, Simone Boeri, Lucia Fanizza, Francesca Sardelli, Lorenzo Jacchetti, Emanuela Raimondi, Manuela T. Rito, Laura Di Craparotta, Ilaria Bolis, Marco Giordano, Carmen Albani, Diego Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title | Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title_full | Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title_fullStr | Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title_full_unstemmed | Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title_short | Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device |
title_sort | human gut epithelium features recapitulated in minerva 2.0 millifluidic organ-on-a-chip device |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511260/ https://www.ncbi.nlm.nih.gov/pubmed/37736017 http://dx.doi.org/10.1063/5.0144862 |
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