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Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps
The release of neutrophil extracellular traps (NETs) can be either beneficial or detrimental for the host, thus it is necessary to maintain a balance between formation and clearance of NETs. Multiple physiological factors eliciting NET release have been identified, yet the studies on natural signals...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511515/ https://www.ncbi.nlm.nih.gov/pubmed/37730741 http://dx.doi.org/10.1038/s41598-023-42167-1 |
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author | Manda-Handzlik, Aneta Cieloch, Adrianna Kuźmicka, Weronika Mroczek, Agnieszka Stelmaszczyk-Emmel, Anna Demkow, Urszula Wachowska, Małgorzata |
author_facet | Manda-Handzlik, Aneta Cieloch, Adrianna Kuźmicka, Weronika Mroczek, Agnieszka Stelmaszczyk-Emmel, Anna Demkow, Urszula Wachowska, Małgorzata |
author_sort | Manda-Handzlik, Aneta |
collection | PubMed |
description | The release of neutrophil extracellular traps (NETs) can be either beneficial or detrimental for the host, thus it is necessary to maintain a balance between formation and clearance of NETs. Multiple physiological factors eliciting NET release have been identified, yet the studies on natural signals limiting NET formation have been scarce. Accordingly, our aim was to analyze whether cytokines or immune cells can inhibit NET formation. To that end, human granulocytes were incubated with interleukin (IL)-4, IL-10, transforming growth factor beta-2 or adenosine and then stimulated to release NETs. Additionally, neutrophils were cultured in the presence of natural killer (NK) cells, regulatory T cells (Tregs), pro-inflammatory or anti-inflammatory macrophages (M1 or M2 macrophages), or in the presence of NK/Tregs/M1 macrophages or M2 macrophages-conditioned medium and subsequently stimulated to release NETs. Our studies showed that secretome of M1 and M2 macrophages, but not of NK cells and Tregs, diminishes NET formation. Co-culture experiments did not reveal any effect of immune cells on NET release. No effect of cytokines or adenosine on NET release was found. This study highlights the importance of paracrine signaling at the site of infection and is the first to show that macrophage secretome can regulate NET formation. |
format | Online Article Text |
id | pubmed-10511515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105115152023-09-22 Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps Manda-Handzlik, Aneta Cieloch, Adrianna Kuźmicka, Weronika Mroczek, Agnieszka Stelmaszczyk-Emmel, Anna Demkow, Urszula Wachowska, Małgorzata Sci Rep Article The release of neutrophil extracellular traps (NETs) can be either beneficial or detrimental for the host, thus it is necessary to maintain a balance between formation and clearance of NETs. Multiple physiological factors eliciting NET release have been identified, yet the studies on natural signals limiting NET formation have been scarce. Accordingly, our aim was to analyze whether cytokines or immune cells can inhibit NET formation. To that end, human granulocytes were incubated with interleukin (IL)-4, IL-10, transforming growth factor beta-2 or adenosine and then stimulated to release NETs. Additionally, neutrophils were cultured in the presence of natural killer (NK) cells, regulatory T cells (Tregs), pro-inflammatory or anti-inflammatory macrophages (M1 or M2 macrophages), or in the presence of NK/Tregs/M1 macrophages or M2 macrophages-conditioned medium and subsequently stimulated to release NETs. Our studies showed that secretome of M1 and M2 macrophages, but not of NK cells and Tregs, diminishes NET formation. Co-culture experiments did not reveal any effect of immune cells on NET release. No effect of cytokines or adenosine on NET release was found. This study highlights the importance of paracrine signaling at the site of infection and is the first to show that macrophage secretome can regulate NET formation. Nature Publishing Group UK 2023-09-20 /pmc/articles/PMC10511515/ /pubmed/37730741 http://dx.doi.org/10.1038/s41598-023-42167-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Manda-Handzlik, Aneta Cieloch, Adrianna Kuźmicka, Weronika Mroczek, Agnieszka Stelmaszczyk-Emmel, Anna Demkow, Urszula Wachowska, Małgorzata Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title | Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title_full | Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title_fullStr | Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title_full_unstemmed | Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title_short | Secretomes of M1 and M2 macrophages decrease the release of neutrophil extracellular traps |
title_sort | secretomes of m1 and m2 macrophages decrease the release of neutrophil extracellular traps |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511515/ https://www.ncbi.nlm.nih.gov/pubmed/37730741 http://dx.doi.org/10.1038/s41598-023-42167-1 |
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