Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila

Capillary Feeder assay (CAFE) is a real-time feeding assay used in Drosophila that employs micro-capillaries, which are costly. Here, we present a modified version of the assay by replacing micro-capillaries with micro-tips, hence ensuring the same principle with cost reduction by 500 times. We deve...

Descripción completa

Detalles Bibliográficos
Autores principales: Segu, Aishwarya, Radhakrishnan, Devika, Kannan, Nisha N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511848/
https://www.ncbi.nlm.nih.gov/pubmed/37384520
http://dx.doi.org/10.1016/j.xpro.2023.102393
Descripción
Sumario:Capillary Feeder assay (CAFE) is a real-time feeding assay used in Drosophila that employs micro-capillaries, which are costly. Here, we present a modified version of the assay by replacing micro-capillaries with micro-tips, hence ensuring the same principle with cost reduction by 500 times. We developed a mathematical approach to measure volume for the conical shaped micro-tips. In this protocol, we describe step-by-step procedures of pre-assay setup along with fly rearing; assay setup included with detailed analysis for volume calculations. For further verification and use of this protocol, please refer to Segu and Kannan.(1)

Ejemplares similares