Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila

Capillary Feeder assay (CAFE) is a real-time feeding assay used in Drosophila that employs micro-capillaries, which are costly. Here, we present a modified version of the assay by replacing micro-capillaries with micro-tips, hence ensuring the same principle with cost reduction by 500 times. We deve...

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Autores principales: Segu, Aishwarya, Radhakrishnan, Devika, Kannan, Nisha N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511848/
https://www.ncbi.nlm.nih.gov/pubmed/37384520
http://dx.doi.org/10.1016/j.xpro.2023.102393
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author Segu, Aishwarya
Radhakrishnan, Devika
Kannan, Nisha N.
author_facet Segu, Aishwarya
Radhakrishnan, Devika
Kannan, Nisha N.
author_sort Segu, Aishwarya
collection PubMed
description Capillary Feeder assay (CAFE) is a real-time feeding assay used in Drosophila that employs micro-capillaries, which are costly. Here, we present a modified version of the assay by replacing micro-capillaries with micro-tips, hence ensuring the same principle with cost reduction by 500 times. We developed a mathematical approach to measure volume for the conical shaped micro-tips. In this protocol, we describe step-by-step procedures of pre-assay setup along with fly rearing; assay setup included with detailed analysis for volume calculations. For further verification and use of this protocol, please refer to Segu and Kannan.(1)
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spelling pubmed-105118482023-09-22 Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila Segu, Aishwarya Radhakrishnan, Devika Kannan, Nisha N. STAR Protoc Protocol Capillary Feeder assay (CAFE) is a real-time feeding assay used in Drosophila that employs micro-capillaries, which are costly. Here, we present a modified version of the assay by replacing micro-capillaries with micro-tips, hence ensuring the same principle with cost reduction by 500 times. We developed a mathematical approach to measure volume for the conical shaped micro-tips. In this protocol, we describe step-by-step procedures of pre-assay setup along with fly rearing; assay setup included with detailed analysis for volume calculations. For further verification and use of this protocol, please refer to Segu and Kannan.(1) Elsevier 2023-06-28 /pmc/articles/PMC10511848/ /pubmed/37384520 http://dx.doi.org/10.1016/j.xpro.2023.102393 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Segu, Aishwarya
Radhakrishnan, Devika
Kannan, Nisha N.
Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title_full Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title_fullStr Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title_full_unstemmed Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title_short Modified Capillary Feeder assay using micro-tips to measure real-time feeding in Drosophila
title_sort modified capillary feeder assay using micro-tips to measure real-time feeding in drosophila
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511848/
https://www.ncbi.nlm.nih.gov/pubmed/37384520
http://dx.doi.org/10.1016/j.xpro.2023.102393
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