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Protocol for generating airway organoids from 2D air liquid interface-differentiated nasal epithelia for use in a functional CFTR assay

We present a protocol to generate organoids from air-liquid-interface (ALI)-differentiated nasal epithelia. We detail their application as cystic fibrosis (CF) disease model in the cystic fibrosis transmembrane conductance regulator (CFTR)-dependent forskolin-induced swelling (FIS) assay. We describ...

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Detalles Bibliográficos
Autores principales:	Rodenburg, Lisa W., van der Windt, Isabelle S., Dreyer, Henriette H.M., Smits, Shannon M.A., den Hertog - Oosterhoff, Loes A., Aarts, Ellen M., Beekman, Jeffrey M., Amatngalim, Gimano D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511861/ https://www.ncbi.nlm.nih.gov/pubmed/37314920 http://dx.doi.org/10.1016/j.xpro.2023.102337

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