Protocol for optimizing production and quality control of infective EcoHIV virions

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral p...

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Detalles Bibliográficos
Autores principales: Alfar, Hammodah R., Pariser, Daphne N., Chanzu, Harry, Joshi, Smita, Coenen, Daniëlle M., Lykins, Joshua, Prakhya, Kanakanagavalli Shravani, Potash, Mary Jane, Chao, Wei, Kelschenbach, Jennifer, Volsky, David J., Metcalf-Pate, Kelly, Whiteheart, Sidney W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511864/
https://www.ncbi.nlm.nih.gov/pubmed/37342907
http://dx.doi.org/10.1016/j.xpro.2023.102368
Descripción
Sumario:EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.

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