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Protocol for optimizing production and quality control of infective EcoHIV virions
EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral p...
| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511864/ https://www.ncbi.nlm.nih.gov/pubmed/37342907 http://dx.doi.org/10.1016/j.xpro.2023.102368 |
| _version_ | 1785108236973637632 |
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| author | Alfar, Hammodah R. Pariser, Daphne N. Chanzu, Harry Joshi, Smita Coenen, Daniëlle M. Lykins, Joshua Prakhya, Kanakanagavalli Shravani Potash, Mary Jane Chao, Wei Kelschenbach, Jennifer Volsky, David J. Metcalf-Pate, Kelly Whiteheart, Sidney W. |
| author_facet | Alfar, Hammodah R. Pariser, Daphne N. Chanzu, Harry Joshi, Smita Coenen, Daniëlle M. Lykins, Joshua Prakhya, Kanakanagavalli Shravani Potash, Mary Jane Chao, Wei Kelschenbach, Jennifer Volsky, David J. Metcalf-Pate, Kelly Whiteheart, Sidney W. |
| author_sort | Alfar, Hammodah R. |
| collection | PubMed |
| description | EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data. |
| format | Online Article Text |
| id | pubmed-10511864 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-105118642023-09-22 Protocol for optimizing production and quality control of infective EcoHIV virions Alfar, Hammodah R. Pariser, Daphne N. Chanzu, Harry Joshi, Smita Coenen, Daniëlle M. Lykins, Joshua Prakhya, Kanakanagavalli Shravani Potash, Mary Jane Chao, Wei Kelschenbach, Jennifer Volsky, David J. Metcalf-Pate, Kelly Whiteheart, Sidney W. STAR Protoc Protocol EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data. Elsevier 2023-06-19 /pmc/articles/PMC10511864/ /pubmed/37342907 http://dx.doi.org/10.1016/j.xpro.2023.102368 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Protocol Alfar, Hammodah R. Pariser, Daphne N. Chanzu, Harry Joshi, Smita Coenen, Daniëlle M. Lykins, Joshua Prakhya, Kanakanagavalli Shravani Potash, Mary Jane Chao, Wei Kelschenbach, Jennifer Volsky, David J. Metcalf-Pate, Kelly Whiteheart, Sidney W. Protocol for optimizing production and quality control of infective EcoHIV virions |
| title | Protocol for optimizing production and quality control of infective EcoHIV virions |
| title_full | Protocol for optimizing production and quality control of infective EcoHIV virions |
| title_fullStr | Protocol for optimizing production and quality control of infective EcoHIV virions |
| title_full_unstemmed | Protocol for optimizing production and quality control of infective EcoHIV virions |
| title_short | Protocol for optimizing production and quality control of infective EcoHIV virions |
| title_sort | protocol for optimizing production and quality control of infective ecohiv virions |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511864/ https://www.ncbi.nlm.nih.gov/pubmed/37342907 http://dx.doi.org/10.1016/j.xpro.2023.102368 |
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