Cargando…

Protocol for optimizing production and quality control of infective EcoHIV virions

EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral p...

Descripción completa

Detalles Bibliográficos
Autores principales:	Alfar, Hammodah R., Pariser, Daphne N., Chanzu, Harry, Joshi, Smita, Coenen, Daniëlle M., Lykins, Joshua, Prakhya, Kanakanagavalli Shravani, Potash, Mary Jane, Chao, Wei, Kelschenbach, Jennifer, Volsky, David J., Metcalf-Pate, Kelly, Whiteheart, Sidney W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511864/ https://www.ncbi.nlm.nih.gov/pubmed/37342907 http://dx.doi.org/10.1016/j.xpro.2023.102368

Ejemplares similares

Buprenorphine reverses neurocognitive impairment in EcoHIV infected mice: A potential therapy for HIV-NCI
por: Murphy, Aniella J., et al.
Publicado: (2022)

Peroxisome Proliferator-Activated Receptor-gamma agonists exhibit anti-inflammatory and antiviral effects in an EcoHIV mouse model
por: Omeragic, Amila, et al.
Publicado: (2019)

A sensitive and adaptable method to measure platelet‐fibrin clot contraction kinetics
por: Prakhya, Kanakanagavalli Shravani, et al.
Publicado: (2022)

Intranasal insulin therapy reverses hippocampal dendritic injury and cognitive impairment in a model of HIV-associated neurocognitive disorders in EcoHIV-infected mice
por: Kim, Boe-Hyun, et al.
Publicado: (2019)

EcoHIV infection of mice establishes latent viral reservoirs in T cells and active viral reservoirs in macrophages that are sufficient for induction of neurocognitive impairment
por: Gu, Chao-Jiang, et al.
Publicado: (2018)

Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury
por: Hubbard, William Brad, et al.
Publicado: (2021)

Glutamine antagonist JHU083 improves psychosocial behavior and sleep deficits in EcoHIV-infected mice
por: Bell, Benjamin J., et al.
Publicado: (2022)

Disrupted Decision-Making: EcoHIV Inoculation in Cocaine Dependent Rats
por: McLaurin, Kristen A., et al.
Publicado: (2022)

Implication of the Autophagy-Related Protein Beclin1 in the Regulation of EcoHIV Replication and Inflammatory Responses
por: Rodriguez, Myosotys, et al.
Publicado: (2023)

Prevention and treatment of HIV infection and cognitive disease in mice by innate immune responses
por: Dong, Baojun, et al.
Publicado: (2020)

CCL2 is required for initiation but not persistence of HIV infection mediated neurocognitive disease in mice
por: Kim, Boe-Hyun, et al.
Publicado: (2023)

A HIV-Tat/C4-binding protein chimera encoded by a DNA vaccine is highly immunogenic and contains acute EcoHIV infection in mice
por: Tomusange, Khamis, et al.
Publicado: (2016)

Inflammation Drives Coagulopathies in Sars-Cov-2 Patients
por: Sim, Martha MS, et al.
Publicado: (2020)

Enhanced Human Immunodeficiency Virus Type 1 Expression and Neuropathogenesis in Knockout Mice Lacking Type I Interferon Responses
por: He, Hongxia, et al.
Publicado: (2014)

Response to ‘Remarks on the article of Hadas et al.: Transmission of chimeric HIV by mating in conventional mice: prevention by pre-exposure antiretroviral therapy and reduced susceptibility during estrus’
por: Potash, Mary Jane, et al.
Publicado: (2014)

Efficient Expression of HIV in Immunocompetent Mouse Brain Reveals a Novel Nonneurotoxic Viral Function in Hippocampal Synaptodendritic Injury and Memory Impairment
por: Kelschenbach, Jennifer, et al.
Publicado: (2019)

Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection
por: Li, Jinliang, et al.
Publicado: (2007)

Virion Structure and Composition
por: Burrell, Christopher J., et al.
Publicado: (2017)

RF_phage virion: Classification of phage virion proteins with a random forest model
por: Zhang, Yanqin, et al.
Publicado: (2023)

Native Morphology of Influenza Virions
por: Noda, Takeshi
Publicado: (2012)

Corrigendum: RF_phage virion: classification of phage virion proteins with a random forest model
por: Zhang, Yanqin, et al.
Publicado: (2023)

An Ensemble Method to Distinguish Bacteriophage Virion from Non-Virion Proteins Based on Protein Sequence Characteristics
por: Zhang, Lina, et al.
Publicado: (2015)

Induction of Potent and Long-Lived Antibody and Cellular Immune Responses in the Genitorectal Mucosa Could be the Critical Determinant of HIV Vaccine Efficacy
por: Chanzu, Nadia, et al.
Publicado: (2014)

Packaging of viral RNAs in virions of adenoviruses
por: Xing, Li, et al.
Publicado: (2009)

Morphogenesis of the Infectious HIV-1 Virion
por: Sakuragi, Jun-ichi
Publicado: (2011)

Antibody-Mediated Immobilization of Virions in Mucus
por: Jensen, Melanie A., et al.
Publicado: (2019)

Histone Modifications in Papillomavirus Virion Minichromosomes
por: Porter, Samuel S., et al.
Publicado: (2021)

On the Sensitivity of the Virion Envelope to Lipid Peroxidation
por: Correa Sierra, Consuelo B., et al.
Publicado: (2022)

Significant Effects of Antiretroviral Therapy on Global Gene Expression in Brain Tissues of Patients with HIV-1-Associated Neurocognitive Disorders
por: Borjabad, Alejandra, et al.
Publicado: (2011)

HIV induces expression of complement component C3 in astrocytes by NF-κB-dependent activation of interleukin-6 synthesis
por: Nitkiewicz, Jadwiga, et al.
Publicado: (2017)

Proteomic analysis of the EhV-86 virion
por: Allen, Michael J, et al.
Publicado: (2008)

A Rondelay (Without Cadenza) By The Virion Of Influenza
por: Kilbourne, Edwin D.
Publicado: (2008)

Retroviral Env Glycoprotein Trafficking and Incorporation into Virions
por: Murakami, Tsutomu
Publicado: (2012)

The Adenovirus Genome Contributes to the Structural Stability of the Virion
por: Saha, Bratati, et al.
Publicado: (2014)

Mechanism of HIV-1 Virion Entrapment by Tetherin
por: Venkatesh, Siddarth, et al.
Publicado: (2013)

Incorporation of Spike and Membrane Glycoproteins into Coronavirus Virions
por: Ujike, Makoto, et al.
Publicado: (2015)

Extracellular Virions: The Advance Guard of Poxvirus Infections
por: Pickup, David J.
Publicado: (2015)

Exploring the inside details of virions by electron microscopy
por: Liu, Zheng, et al.
Publicado: (2016)

The Structure and Chemistry of the Virion: A Systematic Survey
por: FENNER, FRANK, et al.
Publicado: (1974)

Virion Structure, Genome Organization, and Taxonomy of Viruses
por: Fermin, Gustavo
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_tvortn3n3pok05i78pg8th64lf