A protocol for visualization of murine in situ neurovascular interfaces

Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer cast...

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Detalles Bibliográficos
Autores principales: Dang, Danielle D., Chandrashekhar, Vikram, Chandrashekhar, Vibhu, Ghabdanzanluqui, Nagela, Knutsen, Russell H., Nazari, Matthew A., Nimmagadda, Likitha, Donahue, Danielle R., McGavern, Dorian B., Kozel, Beth A., Heiss, John D., Pacak, Karel, Zhuang, Zhengping, Rosenblum, Jared S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511866/
https://www.ncbi.nlm.nih.gov/pubmed/37339049
http://dx.doi.org/10.1016/j.xpro.2023.102367
Descripción
Sumario:Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images. For complete details on the use and execution of this protocol, please refer to Rosenblum et al.(1)

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