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Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints

The rhizomes of Curcuma species have a long medicinal history in Asia. In China, Curcuma species mainly be utilized to make pharmaceutical products, including C. phaecocaulis, C. aromatica, C. wenyujin, C. kwangsiensis and C. longa. In this study, twenty-four samples were selected to study the genet...

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Autores principales: Chen, Mengying, Sun, Jian, Yao, Hui, Gong, Fuyu, Cai, Long, Wang, Chanyan, Shao, Qingsong, Wang, Zhian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511903/
https://www.ncbi.nlm.nih.gov/pubmed/37746000
http://dx.doi.org/10.3389/fpls.2023.1229041
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author Chen, Mengying
Sun, Jian
Yao, Hui
Gong, Fuyu
Cai, Long
Wang, Chanyan
Shao, Qingsong
Wang, Zhian
author_facet Chen, Mengying
Sun, Jian
Yao, Hui
Gong, Fuyu
Cai, Long
Wang, Chanyan
Shao, Qingsong
Wang, Zhian
author_sort Chen, Mengying
collection PubMed
description The rhizomes of Curcuma species have a long medicinal history in Asia. In China, Curcuma species mainly be utilized to make pharmaceutical products, including C. phaecocaulis, C. aromatica, C. wenyujin, C. kwangsiensis and C. longa. In this study, twenty-four samples were selected to study the genetic and chemical variability among five Curcuma species. The ITS2 and trnK intron gene fragment were used to identify the five Curcuma species, the differences in chemical composition were computed using the Euclidean distance based on the data of HPLC characteristic peak areas and the content of six key components, and agronomic characteristics were analyzed including morphological and volatile oil characteristics. The ITS2 and trnK intron gene fragment could distinguish the five Curcuma species clearly. The genetic distance between Curcuma species ranged from 0.0085 to 0.0767 based on the data of ITS2 gene sequences with 32 variation sites, and the genetic distance between Curcuma species ranged from 0.0003 to 0.0194 based on the data of trnK intron gene sequences with 39 variation sites. Five Curcuma species showed otherness chemical composition characteristics, with the Euclidean distance ranging from 3.373 to 6.998. The C. longa showed the biggest variation compared with other species, with the Euclidean distance above 6.239. Among the samples of the original plants of Ezhu, the volatile oil yield of W1 was the highest, reached to 105.75 mL per single plant. Among all the samples, J6 showed the highest yield of volatile oil, reached to 149.42 mL per single plant. The results showed that chemical composition similarity of the medicinal plants was the primary proof for the selection of the original plants of the Curcuma medicinal materials. The genetic distance and chemical variability were important references for discovering new medicinal plant resources.
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spelling pubmed-105119032023-09-22 Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints Chen, Mengying Sun, Jian Yao, Hui Gong, Fuyu Cai, Long Wang, Chanyan Shao, Qingsong Wang, Zhian Front Plant Sci Plant Science The rhizomes of Curcuma species have a long medicinal history in Asia. In China, Curcuma species mainly be utilized to make pharmaceutical products, including C. phaecocaulis, C. aromatica, C. wenyujin, C. kwangsiensis and C. longa. In this study, twenty-four samples were selected to study the genetic and chemical variability among five Curcuma species. The ITS2 and trnK intron gene fragment were used to identify the five Curcuma species, the differences in chemical composition were computed using the Euclidean distance based on the data of HPLC characteristic peak areas and the content of six key components, and agronomic characteristics were analyzed including morphological and volatile oil characteristics. The ITS2 and trnK intron gene fragment could distinguish the five Curcuma species clearly. The genetic distance between Curcuma species ranged from 0.0085 to 0.0767 based on the data of ITS2 gene sequences with 32 variation sites, and the genetic distance between Curcuma species ranged from 0.0003 to 0.0194 based on the data of trnK intron gene sequences with 39 variation sites. Five Curcuma species showed otherness chemical composition characteristics, with the Euclidean distance ranging from 3.373 to 6.998. The C. longa showed the biggest variation compared with other species, with the Euclidean distance above 6.239. Among the samples of the original plants of Ezhu, the volatile oil yield of W1 was the highest, reached to 105.75 mL per single plant. Among all the samples, J6 showed the highest yield of volatile oil, reached to 149.42 mL per single plant. The results showed that chemical composition similarity of the medicinal plants was the primary proof for the selection of the original plants of the Curcuma medicinal materials. The genetic distance and chemical variability were important references for discovering new medicinal plant resources. Frontiers Media S.A. 2023-09-06 /pmc/articles/PMC10511903/ /pubmed/37746000 http://dx.doi.org/10.3389/fpls.2023.1229041 Text en Copyright © 2023 Chen, Sun, Yao, Gong, Cai, Wang, Shao and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chen, Mengying
Sun, Jian
Yao, Hui
Gong, Fuyu
Cai, Long
Wang, Chanyan
Shao, Qingsong
Wang, Zhian
Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title_full Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title_fullStr Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title_full_unstemmed Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title_short Analysis of genetic and chemical variability of five Curcuma species based on DNA barcoding and HPLC fingerprints
title_sort analysis of genetic and chemical variability of five curcuma species based on dna barcoding and hplc fingerprints
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511903/
https://www.ncbi.nlm.nih.gov/pubmed/37746000
http://dx.doi.org/10.3389/fpls.2023.1229041
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