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Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolati...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511906/ https://www.ncbi.nlm.nih.gov/pubmed/37436904 http://dx.doi.org/10.1016/j.xpro.2023.102444 |
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author | Lee, Yu Jin Shin, Kyeong Jin Chan Chae, Young |
author_facet | Lee, Yu Jin Shin, Kyeong Jin Chan Chae, Young |
author_sort | Lee, Yu Jin |
collection | PubMed |
description | Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis. For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).(1) |
format | Online Article Text |
id | pubmed-10511906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105119062023-09-22 Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model Lee, Yu Jin Shin, Kyeong Jin Chan Chae, Young STAR Protoc Protocol Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis. For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).(1) Elsevier 2023-07-11 /pmc/articles/PMC10511906/ /pubmed/37436904 http://dx.doi.org/10.1016/j.xpro.2023.102444 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Lee, Yu Jin Shin, Kyeong Jin Chan Chae, Young Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title_full | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title_fullStr | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title_full_unstemmed | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title_short | Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
title_sort | protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511906/ https://www.ncbi.nlm.nih.gov/pubmed/37436904 http://dx.doi.org/10.1016/j.xpro.2023.102444 |
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