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Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model

Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolati...

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Detalles Bibliográficos
Autores principales: Lee, Yu Jin, Shin, Kyeong Jin, Chan Chae, Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511906/
https://www.ncbi.nlm.nih.gov/pubmed/37436904
http://dx.doi.org/10.1016/j.xpro.2023.102444
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author Lee, Yu Jin
Shin, Kyeong Jin
Chan Chae, Young
author_facet Lee, Yu Jin
Shin, Kyeong Jin
Chan Chae, Young
author_sort Lee, Yu Jin
collection PubMed
description Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis. For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).(1)
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spelling pubmed-105119062023-09-22 Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model Lee, Yu Jin Shin, Kyeong Jin Chan Chae, Young STAR Protoc Protocol Exosomes mediate intracellular communication between cancer cells and the local/distant microenvironment, which promotes systemic dissemination of cancer. Here, we present a protocol for tumor-derived exosome isolation and in vivo metastasis evaluation in a mouse model. We describe steps for isolating and characterizing exosomes, establishing a metastatic mouse model, and injecting exosomes into mouse. We then detail hematoxylin and eosin staining and analysis. This protocol can be used to investigate exosome function and identify unexplored metastatic regulators related to exosome biogenesis. For complete details on the use and execution of this protocol, please refer to Lee et al. (2023).(1) Elsevier 2023-07-11 /pmc/articles/PMC10511906/ /pubmed/37436904 http://dx.doi.org/10.1016/j.xpro.2023.102444 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lee, Yu Jin
Shin, Kyeong Jin
Chan Chae, Young
Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title_full Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title_fullStr Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title_full_unstemmed Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title_short Protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
title_sort protocol for evaluation of tumor-derived exosome-induced cancer cell metastasis in a mouse model
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511906/
https://www.ncbi.nlm.nih.gov/pubmed/37436904
http://dx.doi.org/10.1016/j.xpro.2023.102444
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