Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures

Impaired function of the retinal pigment epithelium (RPE) resembles a hallmark in many retinal diseases; thus, co-cultivation models with RPE and retinal explants are useful to investigate these. Here, we present an easy-to-handle direct co-cultivation protocol, containing a functional, primary RPE...

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Detalles Bibliográficos
Autores principales: Fietz, Agnes, Hurst, Jose, Joachim, Stephanie C., Schnichels, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511932/
https://www.ncbi.nlm.nih.gov/pubmed/37454298
http://dx.doi.org/10.1016/j.xpro.2023.102443
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author Fietz, Agnes
Hurst, Jose
Joachim, Stephanie C.
Schnichels, Sven
author_facet Fietz, Agnes
Hurst, Jose
Joachim, Stephanie C.
Schnichels, Sven
author_sort Fietz, Agnes
collection PubMed
description Impaired function of the retinal pigment epithelium (RPE) resembles a hallmark in many retinal diseases; thus, co-cultivation models with RPE and retinal explants are useful to investigate these. Here, we present an easy-to-handle direct co-cultivation protocol, containing a functional, primary RPE monolayer and retinal explants. We describe in detail steps for establishing the monolayer and the direct co-cultivation. Techniques, which allow users to investigate the integrity and functionality of the RPE monolayer, are presented and the co-cultivation was tested under stress conditions.
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spelling pubmed-105119322023-09-22 Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures Fietz, Agnes Hurst, Jose Joachim, Stephanie C. Schnichels, Sven STAR Protoc Protocol Impaired function of the retinal pigment epithelium (RPE) resembles a hallmark in many retinal diseases; thus, co-cultivation models with RPE and retinal explants are useful to investigate these. Here, we present an easy-to-handle direct co-cultivation protocol, containing a functional, primary RPE monolayer and retinal explants. We describe in detail steps for establishing the monolayer and the direct co-cultivation. Techniques, which allow users to investigate the integrity and functionality of the RPE monolayer, are presented and the co-cultivation was tested under stress conditions. Elsevier 2023-07-16 /pmc/articles/PMC10511932/ /pubmed/37454298 http://dx.doi.org/10.1016/j.xpro.2023.102443 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Fietz, Agnes
Hurst, Jose
Joachim, Stephanie C.
Schnichels, Sven
Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title_full Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title_fullStr Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title_full_unstemmed Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title_short Establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
title_sort establishment of a primary porcine retinal pigment epithelium monolayer to complement retinal ex vivo cultures
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10511932/
https://www.ncbi.nlm.nih.gov/pubmed/37454298
http://dx.doi.org/10.1016/j.xpro.2023.102443
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