Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni

Although nematode genetics was founded on the use of hermaphrodite genetics for studying animal development and behavior, there is a growing need to extend this work to male/female species. One of the most promising species is C. nigoni, because it is so closely related to the model hermaphroditic C...

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Detalles Bibliográficos
Autores principales: Harbin, Jonathan P, Ellis, Ronald E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512056/
https://www.ncbi.nlm.nih.gov/pubmed/37746063
http://dx.doi.org/10.17912/micropub.biology.000968
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author Harbin, Jonathan P
Ellis, Ronald E
author_facet Harbin, Jonathan P
Ellis, Ronald E
author_sort Harbin, Jonathan P
collection PubMed
description Although nematode genetics was founded on the use of hermaphrodite genetics for studying animal development and behavior, there is a growing need to extend this work to male/female species. One of the most promising species is C. nigoni, because it is so closely related to the model hermaphroditic C. briggsae. We present methods for using CRISPR/Cas9 gene editing to create mutations, and techniques for balancing, maintaining and studying these mutations.
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spelling pubmed-105120562023-09-22 Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni Harbin, Jonathan P Ellis, Ronald E MicroPubl Biol Materials and Reagents Although nematode genetics was founded on the use of hermaphrodite genetics for studying animal development and behavior, there is a growing need to extend this work to male/female species. One of the most promising species is C. nigoni, because it is so closely related to the model hermaphroditic C. briggsae. We present methods for using CRISPR/Cas9 gene editing to create mutations, and techniques for balancing, maintaining and studying these mutations. Caltech Library 2023-09-06 /pmc/articles/PMC10512056/ /pubmed/37746063 http://dx.doi.org/10.17912/micropub.biology.000968 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Harbin, Jonathan P
Ellis, Ronald E
Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title_full Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title_fullStr Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title_full_unstemmed Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title_short Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
title_sort efficient production of crispr/cas9 gene knockouts in the male/female nematode caenorhabditis nigoni
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512056/
https://www.ncbi.nlm.nih.gov/pubmed/37746063
http://dx.doi.org/10.17912/micropub.biology.000968
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