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Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach

Microorganisms associated with mosquitoes have fundamental roles, not only in their nutrition, but also in physiological and immunological processes, and in their adaptation to the environment as well. Studies on mosquito hologenomes have increased significantly during the last years, achieving impo...

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Autores principales: Flores, Guillermo A. M., Lopez, Rocio P., Cerrudo, Carolina S., Consolo, V. Fabiana, Berón, Corina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512223/
https://www.ncbi.nlm.nih.gov/pubmed/37746232
http://dx.doi.org/10.3389/ffunb.2022.918052
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author Flores, Guillermo A. M.
Lopez, Rocio P.
Cerrudo, Carolina S.
Consolo, V. Fabiana
Berón, Corina M.
author_facet Flores, Guillermo A. M.
Lopez, Rocio P.
Cerrudo, Carolina S.
Consolo, V. Fabiana
Berón, Corina M.
author_sort Flores, Guillermo A. M.
collection PubMed
description Microorganisms associated with mosquitoes have fundamental roles, not only in their nutrition, but also in physiological and immunological processes, and in their adaptation to the environment as well. Studies on mosquito hologenomes have increased significantly during the last years, achieving important advances in the characterization of the “core bacteriome” of some species of health importance. However, the fungal mycobiome has not been exhaustively researched, especially throughout the life cycle of some hematophagous mosquito species. In this work, the diversity and composition of fungal communities in different developmental stages, sexes, and adult nutrition of Culex quinquefasciatus reared on laboratory conditions were characterized, using internal transcribed spacer high throughput amplicon sequencing. Larvae presented a higher fungal richness, while sucrose-fed males and females showed a similar diversity between them. Blood-fed females presented few operational taxonomic units with an even distribution. Results are consistent with the reduction of larval microbiota after molting, observed for the bacterial microbiome in other mosquito species. The filamentous Ascomycota Penicillium polonicum and Cladosporium sp. were present in all stages of the mosquitoes; in addition, the presence of yeasts in the insects or their subsequent colonization associated with their diet is also discussed. These results suggest that some species of fungi could be essential for the nutrition and development of mosquitoes throughout their life cycle.
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spelling pubmed-105122232023-09-22 Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach Flores, Guillermo A. M. Lopez, Rocio P. Cerrudo, Carolina S. Consolo, V. Fabiana Berón, Corina M. Front Fungal Biol Fungal Biology Microorganisms associated with mosquitoes have fundamental roles, not only in their nutrition, but also in physiological and immunological processes, and in their adaptation to the environment as well. Studies on mosquito hologenomes have increased significantly during the last years, achieving important advances in the characterization of the “core bacteriome” of some species of health importance. However, the fungal mycobiome has not been exhaustively researched, especially throughout the life cycle of some hematophagous mosquito species. In this work, the diversity and composition of fungal communities in different developmental stages, sexes, and adult nutrition of Culex quinquefasciatus reared on laboratory conditions were characterized, using internal transcribed spacer high throughput amplicon sequencing. Larvae presented a higher fungal richness, while sucrose-fed males and females showed a similar diversity between them. Blood-fed females presented few operational taxonomic units with an even distribution. Results are consistent with the reduction of larval microbiota after molting, observed for the bacterial microbiome in other mosquito species. The filamentous Ascomycota Penicillium polonicum and Cladosporium sp. were present in all stages of the mosquitoes; in addition, the presence of yeasts in the insects or their subsequent colonization associated with their diet is also discussed. These results suggest that some species of fungi could be essential for the nutrition and development of mosquitoes throughout their life cycle. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC10512223/ /pubmed/37746232 http://dx.doi.org/10.3389/ffunb.2022.918052 Text en Copyright © 2022 Flores, Lopez, Cerrudo, Consolo and Berón https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Flores, Guillermo A. M.
Lopez, Rocio P.
Cerrudo, Carolina S.
Consolo, V. Fabiana
Berón, Corina M.
Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title_full Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title_fullStr Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title_full_unstemmed Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title_short Culex quinquefasciatus Holobiont: A Fungal Metagenomic Approach
title_sort culex quinquefasciatus holobiont: a fungal metagenomic approach
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512223/
https://www.ncbi.nlm.nih.gov/pubmed/37746232
http://dx.doi.org/10.3389/ffunb.2022.918052
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