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Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes
The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512232/ https://www.ncbi.nlm.nih.gov/pubmed/37744088 http://dx.doi.org/10.3389/ffunb.2021.777474 |
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author | Gerke, Jennifer Köhler, Anna M. Wennrich, Jan-Peer Große, Verena Shao, Lulu Heinrich, Antje K. Bode, Helge B. Chen, Wanping Surup, Frank Braus, Gerhard H. |
author_facet | Gerke, Jennifer Köhler, Anna M. Wennrich, Jan-Peer Große, Verena Shao, Lulu Heinrich, Antje K. Bode, Helge B. Chen, Wanping Surup, Frank Braus, Gerhard H. |
author_sort | Gerke, Jennifer |
collection | PubMed |
description | The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster. |
format | Online Article Text |
id | pubmed-10512232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105122322023-09-22 Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes Gerke, Jennifer Köhler, Anna M. Wennrich, Jan-Peer Große, Verena Shao, Lulu Heinrich, Antje K. Bode, Helge B. Chen, Wanping Surup, Frank Braus, Gerhard H. Front Fungal Biol Fungal Biology The soil microbiome comprises numerous filamentous fungi and bacteria that mutually react and challenge each other by the production of bioactive secondary metabolites. Herein, we show in liquid co-cultures that the presence of filamentous Streptomycetes producing antifungal glycopeptide antibiotics induces the production of the antibacterial and iron-chelating tropolones anhydrosepedonin (1) and antibiotic C (2) in the mold Aspergillus nidulans. Additionally, the biosynthesis of the related polyketide tripyrnidone (5) was induced, whose novel tricyclic scaffold we elucidated by NMR and HRESIMS data. The corresponding biosynthetic polyketide synthase-encoding gene cluster responsible for the production of these compounds was identified. The tropolones as well as tripyrnidone (5) are produced by genes that belong to the broad reservoir of the fungal genome for the synthesis of different secondary metabolites, which are usually silenced under standard laboratory conditions. These molecules might be part of the bacterium-fungus competition in the complex soil environment, with the bacterial glycopeptide antibiotic as specific environmental trigger for fungal induction of this cluster. Frontiers Media S.A. 2022-01-03 /pmc/articles/PMC10512232/ /pubmed/37744088 http://dx.doi.org/10.3389/ffunb.2021.777474 Text en Copyright © 2022 Gerke, Köhler, Wennrich, Große, Shao, Heinrich, Bode, Chen, Surup and Braus. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Fungal Biology Gerke, Jennifer Köhler, Anna M. Wennrich, Jan-Peer Große, Verena Shao, Lulu Heinrich, Antje K. Bode, Helge B. Chen, Wanping Surup, Frank Braus, Gerhard H. Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title | Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title_full | Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title_fullStr | Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title_full_unstemmed | Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title_short | Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes |
title_sort | biosynthesis of antibacterial iron-chelating tropolones in aspergillus nidulans as response to glycopeptide-producing streptomycetes |
topic | Fungal Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512232/ https://www.ncbi.nlm.nih.gov/pubmed/37744088 http://dx.doi.org/10.3389/ffunb.2021.777474 |
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