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Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus

The button mushroom Agaricus bisporus is represented mainly by two varieties, a secondarily homothallic variety with predominantly two heterokaryotic spores per basidia and a heterothallic variety with predominantly four homokaryotic spored basidium. Both varieties also differ in their recombination...

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Autores principales: Sonnenberg, Anton S. M., Sedaghat-Telgerd, Narges, Lavrijssen, Brian, Hendrickx, Patrick M., Scholtmeijer, Karin, Baars, Johan J. P., Visser, Richard G. F., van Peer, Arend
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512247/
https://www.ncbi.nlm.nih.gov/pubmed/37744108
http://dx.doi.org/10.3389/ffunb.2021.711330
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author Sonnenberg, Anton S. M.
Sedaghat-Telgerd, Narges
Lavrijssen, Brian
Hendrickx, Patrick M.
Scholtmeijer, Karin
Baars, Johan J. P.
Visser, Richard G. F.
van Peer, Arend
author_facet Sonnenberg, Anton S. M.
Sedaghat-Telgerd, Narges
Lavrijssen, Brian
Hendrickx, Patrick M.
Scholtmeijer, Karin
Baars, Johan J. P.
Visser, Richard G. F.
van Peer, Arend
author_sort Sonnenberg, Anton S. M.
collection PubMed
description The button mushroom Agaricus bisporus is represented mainly by two varieties, a secondarily homothallic variety with predominantly two heterokaryotic spores per basidia and a heterothallic variety with predominantly four homokaryotic spored basidium. Both varieties also differ in their recombination landscape with the former showing crossovers (CO) predominantly at chromosome ends whereas the latter has a more evenly distribution of CO over the chromosomes. The two varieties are compatible, and this has been used to study segregation of the basidial spore number (BSN) and the genomic positions of recombination, i.e., the CO landscape, in order to find the underlying genetic determinants. Knowledge on genes controlling CO positions might facilitate either the conservation of favorable allele combinations or the disruption of unwanted allele combinations to reduce linkage drag. For BSN, in total seven QTL were found with the major QTL on chromosome 1 explaining ca. 55% of the phenotypic variation. It appeared, however, difficult to map the recombination landscape. This phenotype can only be assessed in the meiotic offspring of an intervarietal hybrid which is a laborious and difficult task. Nevertheless, this was done, and we were able to map three QTLs for this trait, two on chromosome 1 and one on chromosome 2 not overlapping with the QTL for BSN. The hurdles encountered are discussed and a new strategy is proposed that can solves these. We propose to use two genetically unrelated mapping populations both offspring of a cross between a var. bisporus and a var. burnettii homokaryon and thus segregating both for CO and BSN. Homokaryotic offspring of both populations can be intercrossed without limitation of mating incompatibility and marker homozygosity and the hybrid mushrooms directly used to map BSN. Homokaryotic offspring of these hybrid mushrooms can be genotypes to assess CO positions using next generation sequencing technologies that will solve marker problems encountered, especially for genotyping chromosome ends. This new approach can be a useful strategy for a more efficient breeding strategy for mushrooms in general.
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spelling pubmed-105122472023-09-22 Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus Sonnenberg, Anton S. M. Sedaghat-Telgerd, Narges Lavrijssen, Brian Hendrickx, Patrick M. Scholtmeijer, Karin Baars, Johan J. P. Visser, Richard G. F. van Peer, Arend Front Fungal Biol Fungal Biology The button mushroom Agaricus bisporus is represented mainly by two varieties, a secondarily homothallic variety with predominantly two heterokaryotic spores per basidia and a heterothallic variety with predominantly four homokaryotic spored basidium. Both varieties also differ in their recombination landscape with the former showing crossovers (CO) predominantly at chromosome ends whereas the latter has a more evenly distribution of CO over the chromosomes. The two varieties are compatible, and this has been used to study segregation of the basidial spore number (BSN) and the genomic positions of recombination, i.e., the CO landscape, in order to find the underlying genetic determinants. Knowledge on genes controlling CO positions might facilitate either the conservation of favorable allele combinations or the disruption of unwanted allele combinations to reduce linkage drag. For BSN, in total seven QTL were found with the major QTL on chromosome 1 explaining ca. 55% of the phenotypic variation. It appeared, however, difficult to map the recombination landscape. This phenotype can only be assessed in the meiotic offspring of an intervarietal hybrid which is a laborious and difficult task. Nevertheless, this was done, and we were able to map three QTLs for this trait, two on chromosome 1 and one on chromosome 2 not overlapping with the QTL for BSN. The hurdles encountered are discussed and a new strategy is proposed that can solves these. We propose to use two genetically unrelated mapping populations both offspring of a cross between a var. bisporus and a var. burnettii homokaryon and thus segregating both for CO and BSN. Homokaryotic offspring of both populations can be intercrossed without limitation of mating incompatibility and marker homozygosity and the hybrid mushrooms directly used to map BSN. Homokaryotic offspring of these hybrid mushrooms can be genotypes to assess CO positions using next generation sequencing technologies that will solve marker problems encountered, especially for genotyping chromosome ends. This new approach can be a useful strategy for a more efficient breeding strategy for mushrooms in general. Frontiers Media S.A. 2021-08-20 /pmc/articles/PMC10512247/ /pubmed/37744108 http://dx.doi.org/10.3389/ffunb.2021.711330 Text en Copyright © 2021 Sonnenberg, Sedaghat-Telgerd, Lavrijssen, Hendrickx, Scholtmeijer, Baars, Visser and van Peer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Sonnenberg, Anton S. M.
Sedaghat-Telgerd, Narges
Lavrijssen, Brian
Hendrickx, Patrick M.
Scholtmeijer, Karin
Baars, Johan J. P.
Visser, Richard G. F.
van Peer, Arend
Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title_full Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title_fullStr Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title_full_unstemmed Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title_short Mapping Recombination Landscape and Basidial Spore Number in the Button Mushroom Agaricus bisporus
title_sort mapping recombination landscape and basidial spore number in the button mushroom agaricus bisporus
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512247/
https://www.ncbi.nlm.nih.gov/pubmed/37744108
http://dx.doi.org/10.3389/ffunb.2021.711330
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