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Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces

Fusarium verticillioides is a mycotoxigenic fungus that is a threat to food and feed safety due to its common infection of maize, a global staple crop. A proposed strategy to combat this threat is the use of biological control bacteria that can inhibit the fungus and reduce mycotoxin contamination....

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Autores principales: Satterlee, Timothy R., Williams, Felicia N., Nadal, Marina, Glenn, Anthony E., Lofton, Lily W., Duke, Mary V., Scheffler, Brian E., Gold, Scott E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512263/
https://www.ncbi.nlm.nih.gov/pubmed/37746240
http://dx.doi.org/10.3389/ffunb.2022.894590
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author Satterlee, Timothy R.
Williams, Felicia N.
Nadal, Marina
Glenn, Anthony E.
Lofton, Lily W.
Duke, Mary V.
Scheffler, Brian E.
Gold, Scott E.
author_facet Satterlee, Timothy R.
Williams, Felicia N.
Nadal, Marina
Glenn, Anthony E.
Lofton, Lily W.
Duke, Mary V.
Scheffler, Brian E.
Gold, Scott E.
author_sort Satterlee, Timothy R.
collection PubMed
description Fusarium verticillioides is a mycotoxigenic fungus that is a threat to food and feed safety due to its common infection of maize, a global staple crop. A proposed strategy to combat this threat is the use of biological control bacteria that can inhibit the fungus and reduce mycotoxin contamination. In this study, the effect of multiple environmental isolates of Streptomyces on F. verticillioides was examined via transcriptome analysis. The Streptomyces strains ranged from inducing no visible response to dramatic growth inhibition. Transcriptionally, F. verticillioides responded proportionally to strain inhibition with either little to no transcript changes to thousands of genes being differentially expressed. Expression changes in multiple F. verticillioides putative secondary metabolite gene clusters was observed. Interestingly, genes involved in the fusaric acid gene cluster were suppressed by inhibitory strains of Streptomyces. A F. verticillioides beta-lactamase encoding gene (FVEG_13172) was found to be highly induced by specific inhibitory Streptomyces strains and its deletion increased visible response to those strains. This study demonstrates that F. verticillioides does not have an all or nothing response to bacteria it encounters but rather a measured response that is strain specific and proportional to the strength of inhibition.
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spelling pubmed-105122632023-09-22 Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces Satterlee, Timothy R. Williams, Felicia N. Nadal, Marina Glenn, Anthony E. Lofton, Lily W. Duke, Mary V. Scheffler, Brian E. Gold, Scott E. Front Fungal Biol Fungal Biology Fusarium verticillioides is a mycotoxigenic fungus that is a threat to food and feed safety due to its common infection of maize, a global staple crop. A proposed strategy to combat this threat is the use of biological control bacteria that can inhibit the fungus and reduce mycotoxin contamination. In this study, the effect of multiple environmental isolates of Streptomyces on F. verticillioides was examined via transcriptome analysis. The Streptomyces strains ranged from inducing no visible response to dramatic growth inhibition. Transcriptionally, F. verticillioides responded proportionally to strain inhibition with either little to no transcript changes to thousands of genes being differentially expressed. Expression changes in multiple F. verticillioides putative secondary metabolite gene clusters was observed. Interestingly, genes involved in the fusaric acid gene cluster were suppressed by inhibitory strains of Streptomyces. A F. verticillioides beta-lactamase encoding gene (FVEG_13172) was found to be highly induced by specific inhibitory Streptomyces strains and its deletion increased visible response to those strains. This study demonstrates that F. verticillioides does not have an all or nothing response to bacteria it encounters but rather a measured response that is strain specific and proportional to the strength of inhibition. Frontiers Media S.A. 2022-07-28 /pmc/articles/PMC10512263/ /pubmed/37746240 http://dx.doi.org/10.3389/ffunb.2022.894590 Text en Copyright © 2022 Satterlee, Williams, Nadal, Glenn, Lofton, Duke, Scheffler and Gold https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Satterlee, Timothy R.
Williams, Felicia N.
Nadal, Marina
Glenn, Anthony E.
Lofton, Lily W.
Duke, Mary V.
Scheffler, Brian E.
Gold, Scott E.
Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title_full Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title_fullStr Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title_full_unstemmed Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title_short Transcriptomic Response of Fusarium verticillioides to Variably Inhibitory Environmental Isolates of Streptomyces
title_sort transcriptomic response of fusarium verticillioides to variably inhibitory environmental isolates of streptomyces
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512263/
https://www.ncbi.nlm.nih.gov/pubmed/37746240
http://dx.doi.org/10.3389/ffunb.2022.894590
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