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UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil

Metarhizium comprises a phylogenetically diverse genus of entomopathogenic fungi. In Brazil, Metarhizium anisopliae s.str. subclade Mani 2 is predominantly isolated from insects, while M. robertsii and M. brunneum mostly occur in the soil environment. Solar radiation and high temperatures are import...

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Autores principales: Couceiro, Joel da Cruz, Fatoretto, Maíra Blumer, Demétrio, Clarice Garcia Borges, Meyling, Nicolai Vitt, Delalibera, Ítalo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512313/
https://www.ncbi.nlm.nih.gov/pubmed/37744102
http://dx.doi.org/10.3389/ffunb.2021.645737
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author Couceiro, Joel da Cruz
Fatoretto, Maíra Blumer
Demétrio, Clarice Garcia Borges
Meyling, Nicolai Vitt
Delalibera, Ítalo
author_facet Couceiro, Joel da Cruz
Fatoretto, Maíra Blumer
Demétrio, Clarice Garcia Borges
Meyling, Nicolai Vitt
Delalibera, Ítalo
author_sort Couceiro, Joel da Cruz
collection PubMed
description Metarhizium comprises a phylogenetically diverse genus of entomopathogenic fungi. In Brazil, Metarhizium anisopliae s.str. subclade Mani 2 is predominantly isolated from insects, while M. robertsii and M. brunneum mostly occur in the soil environment. Solar radiation and high temperatures are important abiotic factors that can be detrimental to fungal propagules. We hypothesized that among 12 Brazilian isolates of Metarhizium spp., M. anisopliae Mani 2 (n = 6), being adapted to abiotic conditions of the phylloplane, is more tolerant to UV light and high temperatures than M. robertsii (n = 3) and M. brunneum (n = 3). Inoculum of each isolate was exposed to UV-B for up to 8 h and viability evaluated 48 h later. After 8 h under UV-B, most of the isolates had germination rates below 5%. Discs of mycelia were incubated at different temperatures, and diameter of colonies were recorded for 12 days. Mycelia of M. robertsii isolates grew faster at 33 °C, while M. anisopliae and M. brunneum grew most at 25 °C. Dry conidia were incubated at 20, 25 or 40 °C for 12 days, and then viabilities were examined. At 40 °C, conidia of five M. anisopliae isolates were the most tolerant. In the three experiments, considerable intra- and inter-specific variability was detected. The results indicate that conclusions about tolerance to these abiotic factors should be made only at the isolate level.
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spelling pubmed-105123132023-09-22 UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil Couceiro, Joel da Cruz Fatoretto, Maíra Blumer Demétrio, Clarice Garcia Borges Meyling, Nicolai Vitt Delalibera, Ítalo Front Fungal Biol Fungal Biology Metarhizium comprises a phylogenetically diverse genus of entomopathogenic fungi. In Brazil, Metarhizium anisopliae s.str. subclade Mani 2 is predominantly isolated from insects, while M. robertsii and M. brunneum mostly occur in the soil environment. Solar radiation and high temperatures are important abiotic factors that can be detrimental to fungal propagules. We hypothesized that among 12 Brazilian isolates of Metarhizium spp., M. anisopliae Mani 2 (n = 6), being adapted to abiotic conditions of the phylloplane, is more tolerant to UV light and high temperatures than M. robertsii (n = 3) and M. brunneum (n = 3). Inoculum of each isolate was exposed to UV-B for up to 8 h and viability evaluated 48 h later. After 8 h under UV-B, most of the isolates had germination rates below 5%. Discs of mycelia were incubated at different temperatures, and diameter of colonies were recorded for 12 days. Mycelia of M. robertsii isolates grew faster at 33 °C, while M. anisopliae and M. brunneum grew most at 25 °C. Dry conidia were incubated at 20, 25 or 40 °C for 12 days, and then viabilities were examined. At 40 °C, conidia of five M. anisopliae isolates were the most tolerant. In the three experiments, considerable intra- and inter-specific variability was detected. The results indicate that conclusions about tolerance to these abiotic factors should be made only at the isolate level. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC10512313/ /pubmed/37744102 http://dx.doi.org/10.3389/ffunb.2021.645737 Text en Copyright © 2021 Couceiro, Fatoretto, Demétrio, Meyling and Delalibera https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Couceiro, Joel da Cruz
Fatoretto, Maíra Blumer
Demétrio, Clarice Garcia Borges
Meyling, Nicolai Vitt
Delalibera, Ítalo
UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title_full UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title_fullStr UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title_full_unstemmed UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title_short UV-B Radiation Tolerance and Temperature-Dependent Activity Within the Entomopathogenic Fungal Genus Metarhizium in Brazil
title_sort uv-b radiation tolerance and temperature-dependent activity within the entomopathogenic fungal genus metarhizium in brazil
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512313/
https://www.ncbi.nlm.nih.gov/pubmed/37744102
http://dx.doi.org/10.3389/ffunb.2021.645737
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