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Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi

The study of evolution is limited by the techniques available to do so. Aside from the use of the fossil record, molecular phylogenetics can provide a detailed characterization of evolutionary histories using genes, genomes and proteins. However, these tools provide scarce biochemical information of...

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Autores principales: Ayuso-Fernández, Iván, Molpeceres, Gonzalo, Camarero, Susana, Ruiz-Dueñas, Francisco Javier, Martínez, Angel T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512382/
https://www.ncbi.nlm.nih.gov/pubmed/37746217
http://dx.doi.org/10.3389/ffunb.2022.1003489
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author Ayuso-Fernández, Iván
Molpeceres, Gonzalo
Camarero, Susana
Ruiz-Dueñas, Francisco Javier
Martínez, Angel T.
author_facet Ayuso-Fernández, Iván
Molpeceres, Gonzalo
Camarero, Susana
Ruiz-Dueñas, Francisco Javier
Martínez, Angel T.
author_sort Ayuso-Fernández, Iván
collection PubMed
description The study of evolution is limited by the techniques available to do so. Aside from the use of the fossil record, molecular phylogenetics can provide a detailed characterization of evolutionary histories using genes, genomes and proteins. However, these tools provide scarce biochemical information of the organisms and systems of interest and are therefore very limited when they come to explain protein evolution. In the past decade, this limitation has been overcome by the development of ancestral sequence reconstruction (ASR) methods. ASR allows the subsequent resurrection in the laboratory of inferred proteins from now extinct organisms, becoming an outstanding tool to study enzyme evolution. Here we review the recent advances in ASR methods and their application to study fungal evolution, with special focus on wood-decay fungi as essential organisms in the global carbon cycling.
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spelling pubmed-105123822023-09-22 Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi Ayuso-Fernández, Iván Molpeceres, Gonzalo Camarero, Susana Ruiz-Dueñas, Francisco Javier Martínez, Angel T. Front Fungal Biol Fungal Biology The study of evolution is limited by the techniques available to do so. Aside from the use of the fossil record, molecular phylogenetics can provide a detailed characterization of evolutionary histories using genes, genomes and proteins. However, these tools provide scarce biochemical information of the organisms and systems of interest and are therefore very limited when they come to explain protein evolution. In the past decade, this limitation has been overcome by the development of ancestral sequence reconstruction (ASR) methods. ASR allows the subsequent resurrection in the laboratory of inferred proteins from now extinct organisms, becoming an outstanding tool to study enzyme evolution. Here we review the recent advances in ASR methods and their application to study fungal evolution, with special focus on wood-decay fungi as essential organisms in the global carbon cycling. Frontiers Media S.A. 2022-10-14 /pmc/articles/PMC10512382/ /pubmed/37746217 http://dx.doi.org/10.3389/ffunb.2022.1003489 Text en Copyright © 2022 Ayuso-Fernández, Molpeceres, Camarero, Ruiz-Dueñas and Martínez https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Ayuso-Fernández, Iván
Molpeceres, Gonzalo
Camarero, Susana
Ruiz-Dueñas, Francisco Javier
Martínez, Angel T.
Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title_full Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title_fullStr Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title_full_unstemmed Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title_short Ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
title_sort ancestral sequence reconstruction as a tool to study the evolution of wood decaying fungi
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512382/
https://www.ncbi.nlm.nih.gov/pubmed/37746217
http://dx.doi.org/10.3389/ffunb.2022.1003489
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