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The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii

Protein ubiquitination, which is involved in various biological processes in eukaryotic cells, is a reversible modification of proteins. Deubiquitinases can maintain ubiquitin homeostasis by removing ubiquitin or modulating protein degradation via the ubiquitin-proteasome system (UPS). Metarhizium r...

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Autores principales: Wang, Zhangxun, Chen, Hua, Li, Hao, Chen, Hanyuan, Huang, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512391/
https://www.ncbi.nlm.nih.gov/pubmed/37746165
http://dx.doi.org/10.3389/ffunb.2022.896466
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author Wang, Zhangxun
Chen, Hua
Li, Hao
Chen, Hanyuan
Huang, Bo
author_facet Wang, Zhangxun
Chen, Hua
Li, Hao
Chen, Hanyuan
Huang, Bo
author_sort Wang, Zhangxun
collection PubMed
description Protein ubiquitination, which is involved in various biological processes in eukaryotic cells, is a reversible modification of proteins. Deubiquitinases can maintain ubiquitin homeostasis by removing ubiquitin or modulating protein degradation via the ubiquitin-proteasome system (UPS). Metarhizium robertsii, an entomopathogenic fungus, has become a model fungus for investigating the interactions between insects and fungal pathogens. To explore the possible effects of the deubiquitination process on the development, stress response, and virulence of M. robertsii, disruption of MrUbp14 (an ortholog of the yeast ubiquitin-specific protease gene, Ubp14) was performed. The results of this study showed that the deletion of MrUbp14 led to accelerated conidial germination, reduced conidial yields, and decreased expression levels of some genes involved in conidiation. Furthermore, the MrUbp14 mutant (ΔMrUbp14) exhibited decreased tolerance to cell wall-damaging stressors (Congo red and SDS) and heat stress. Importantly, the results of the bioassay demonstrated that the fungal virulence of the ΔMrUbp14 strain was largely reduced in cuticle infection, but not in direct injection, which was accompanied by a significant decline in appressorium formation and cuticle penetration. Moreover, our results demonstrated that the disruption of MrUbp14 resulted in significantly increased ubiquitination levels of total protein, suggesting that MrUbp14 acts as a deubiquitinating enzyme in M. robertsii. In summary, our phenotypic changes in the gene disruption mutants suggest that MrUbp14 is important for conidiation, stress response, and fungal virulence in M. robertsii.
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spelling pubmed-105123912023-09-22 The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii Wang, Zhangxun Chen, Hua Li, Hao Chen, Hanyuan Huang, Bo Front Fungal Biol Fungal Biology Protein ubiquitination, which is involved in various biological processes in eukaryotic cells, is a reversible modification of proteins. Deubiquitinases can maintain ubiquitin homeostasis by removing ubiquitin or modulating protein degradation via the ubiquitin-proteasome system (UPS). Metarhizium robertsii, an entomopathogenic fungus, has become a model fungus for investigating the interactions between insects and fungal pathogens. To explore the possible effects of the deubiquitination process on the development, stress response, and virulence of M. robertsii, disruption of MrUbp14 (an ortholog of the yeast ubiquitin-specific protease gene, Ubp14) was performed. The results of this study showed that the deletion of MrUbp14 led to accelerated conidial germination, reduced conidial yields, and decreased expression levels of some genes involved in conidiation. Furthermore, the MrUbp14 mutant (ΔMrUbp14) exhibited decreased tolerance to cell wall-damaging stressors (Congo red and SDS) and heat stress. Importantly, the results of the bioassay demonstrated that the fungal virulence of the ΔMrUbp14 strain was largely reduced in cuticle infection, but not in direct injection, which was accompanied by a significant decline in appressorium formation and cuticle penetration. Moreover, our results demonstrated that the disruption of MrUbp14 resulted in significantly increased ubiquitination levels of total protein, suggesting that MrUbp14 acts as a deubiquitinating enzyme in M. robertsii. In summary, our phenotypic changes in the gene disruption mutants suggest that MrUbp14 is important for conidiation, stress response, and fungal virulence in M. robertsii. Frontiers Media S.A. 2022-05-16 /pmc/articles/PMC10512391/ /pubmed/37746165 http://dx.doi.org/10.3389/ffunb.2022.896466 Text en Copyright © 2022 Wang, Chen, Li, Chen and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Wang, Zhangxun
Chen, Hua
Li, Hao
Chen, Hanyuan
Huang, Bo
The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title_full The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title_fullStr The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title_full_unstemmed The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title_short The Deubiquitinating Enzyme MrUbp14 Is Involved in Conidiation, Stress Response, and Pathogenicity in Metarhizium robertsii
title_sort deubiquitinating enzyme mrubp14 is involved in conidiation, stress response, and pathogenicity in metarhizium robertsii
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512391/
https://www.ncbi.nlm.nih.gov/pubmed/37746165
http://dx.doi.org/10.3389/ffunb.2022.896466
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