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Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes

Fungi are an attractive food source for predators such as fungivorous nematodes. Several fungal defense proteins and their protective mechanisms against nematodes have been described. Many of these proteins are lectins which are stored in the cytoplasm of the fungal cells and bind to specific glycan...

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Autores principales: Tayyrov, Annageldi, Wei, Chunyue, Fetz, Céline, Goryachkin, Aleksandr, Schächle, Philipp, Nyström, Laura, Künzler, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512399/
https://www.ncbi.nlm.nih.gov/pubmed/37744157
http://dx.doi.org/10.3389/ffunb.2021.696972
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author Tayyrov, Annageldi
Wei, Chunyue
Fetz, Céline
Goryachkin, Aleksandr
Schächle, Philipp
Nyström, Laura
Künzler, Markus
author_facet Tayyrov, Annageldi
Wei, Chunyue
Fetz, Céline
Goryachkin, Aleksandr
Schächle, Philipp
Nyström, Laura
Künzler, Markus
author_sort Tayyrov, Annageldi
collection PubMed
description Fungi are an attractive food source for predators such as fungivorous nematodes. Several fungal defense proteins and their protective mechanisms against nematodes have been described. Many of these proteins are lectins which are stored in the cytoplasm of the fungal cells and bind to specific glycan epitopes in the digestive tract of the nematode upon ingestion. Here, we studied two novel nematotoxic proteins with lipase domains from the model mushroom Coprinopsis cinerea. These cytoplasmically localized proteins were found to be induced in the vegetative mycelium of C. cinerea upon challenge with fungivorous nematode Aphelenchus avenae. The proteins showed nematotoxicity when heterologously expressed in E. coli and fed to several bacterivorous nematodes. Site-specific mutagenesis of predicted catalytic residues eliminated the in-vitro lipase activity of the proteins and significantly reduced their nematotoxicity, indicating the importance of the lipase activity for the nematotoxicity of these proteins. Our results suggest that cytoplasmic lipases constitute a novel class of fungal defense proteins against predatory nematodes. These findings improve our understanding of fungal defense mechanisms against predators and may find applications in the control of parasitic nematodes in agriculture and medicine.
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spelling pubmed-105123992023-09-22 Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes Tayyrov, Annageldi Wei, Chunyue Fetz, Céline Goryachkin, Aleksandr Schächle, Philipp Nyström, Laura Künzler, Markus Front Fungal Biol Fungal Biology Fungi are an attractive food source for predators such as fungivorous nematodes. Several fungal defense proteins and their protective mechanisms against nematodes have been described. Many of these proteins are lectins which are stored in the cytoplasm of the fungal cells and bind to specific glycan epitopes in the digestive tract of the nematode upon ingestion. Here, we studied two novel nematotoxic proteins with lipase domains from the model mushroom Coprinopsis cinerea. These cytoplasmically localized proteins were found to be induced in the vegetative mycelium of C. cinerea upon challenge with fungivorous nematode Aphelenchus avenae. The proteins showed nematotoxicity when heterologously expressed in E. coli and fed to several bacterivorous nematodes. Site-specific mutagenesis of predicted catalytic residues eliminated the in-vitro lipase activity of the proteins and significantly reduced their nematotoxicity, indicating the importance of the lipase activity for the nematotoxicity of these proteins. Our results suggest that cytoplasmic lipases constitute a novel class of fungal defense proteins against predatory nematodes. These findings improve our understanding of fungal defense mechanisms against predators and may find applications in the control of parasitic nematodes in agriculture and medicine. Frontiers Media S.A. 2021-07-01 /pmc/articles/PMC10512399/ /pubmed/37744157 http://dx.doi.org/10.3389/ffunb.2021.696972 Text en Copyright © 2021 Tayyrov, Wei, Fetz, Goryachkin, Schächle, Nyström and Künzler. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Fungal Biology
Tayyrov, Annageldi
Wei, Chunyue
Fetz, Céline
Goryachkin, Aleksandr
Schächle, Philipp
Nyström, Laura
Künzler, Markus
Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title_full Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title_fullStr Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title_full_unstemmed Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title_short Cytoplasmic Lipases—A Novel Class of Fungal Defense Proteins Against Nematodes
title_sort cytoplasmic lipases—a novel class of fungal defense proteins against nematodes
topic Fungal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512399/
https://www.ncbi.nlm.nih.gov/pubmed/37744157
http://dx.doi.org/10.3389/ffunb.2021.696972
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