The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation

BACKGROUND: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized f...

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Autores principales: Zhou, Zhi, Zhang, Yong, Tan, Can, Zhang, Juan, Yi, Guohui, Wang, Bangbei, Li, Yejuan, Lu, Hui, Lu, Weiying, Zhang, Xiaopo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512487/
https://www.ncbi.nlm.nih.gov/pubmed/37735639
http://dx.doi.org/10.1186/s12905-023-02632-5
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author Zhou, Zhi
Zhang, Yong
Tan, Can
Zhang, Juan
Yi, Guohui
Wang, Bangbei
Li, Yejuan
Lu, Hui
Lu, Weiying
Zhang, Xiaopo
author_facet Zhou, Zhi
Zhang, Yong
Tan, Can
Zhang, Juan
Yi, Guohui
Wang, Bangbei
Li, Yejuan
Lu, Hui
Lu, Weiying
Zhang, Xiaopo
author_sort Zhou, Zhi
collection PubMed
description BACKGROUND: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. METHODS: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson’ correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. RESULTS: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. CONCLUSION: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12905-023-02632-5.
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spelling pubmed-105124872023-09-22 The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation Zhou, Zhi Zhang, Yong Tan, Can Zhang, Juan Yi, Guohui Wang, Bangbei Li, Yejuan Lu, Hui Lu, Weiying Zhang, Xiaopo BMC Womens Health Research BACKGROUND: MicroRNA-19b (miR-19b) has been reported to be downregulated in polycystic ovary syndrome (PCOS), while its upstream regulators are unclear. We speculated that miR-19b could potentially form a binding relationship with BBOX1 antisense RNA 1 (BBOX1-AS1), a long non-coding RNA recognized for its critical role in ovarian cancer. Subsequently, we investigated into their interaction in PCOS. METHODS: The expression of miR-19b and BBOX1-AS1 in follicular fluid from both control women (n = 80) and women with PCOS (n = 80) was detected by RT-qPCR. Correlations were analyzed with Pearson’ correlation coefficient. The binding of miR-19b to the wild-type (-wt) ad mutant (-mut) BBOX1-AS1 was determined by RNA-RNA pulldown assay. Their interactions were detected by overexpression assay. Bromodeoxyuridine (BrdU) assay was applied for proliferation analysis. RESULTS: BBOX1-AS1 was highly upregulated, while miR-19b was downregulated in PCOS. There was no close correlation across PCOS and the control samples. Consistently, they did not regulate the expression of each other in granulosa cells. However, BBOX1-AS1-wt, but not BBOX1-AS1-mut, could directly interact with miR-19b. BBOX1-AS1 suppressed the role of miR-19b in inhibiting granulosa cell proliferation. CONCLUSION: BBOX1-AS1 is highly upregulated in PCOS, and it may serve as an endogenous competing RNA for miR-19b to suppress its role in inhibiting granulosa cell proliferation. Our study suggested the role of BBOX1-AS1 as a potential target to treat PCOS. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12905-023-02632-5. BioMed Central 2023-09-21 /pmc/articles/PMC10512487/ /pubmed/37735639 http://dx.doi.org/10.1186/s12905-023-02632-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhou, Zhi
Zhang, Yong
Tan, Can
Zhang, Juan
Yi, Guohui
Wang, Bangbei
Li, Yejuan
Lu, Hui
Lu, Weiying
Zhang, Xiaopo
The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title_full The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title_fullStr The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title_full_unstemmed The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title_short The long non-coding RNA BBOX1 antisense RNA 1 is upregulated in polycystic ovary syndrome (PCOS) and suppresses the role of microRNA-19b in the proliferation of ovarian granulose cells: Short title: BBOX1 antisense RNA 1 in cell proliferation
title_sort long non-coding rna bbox1 antisense rna 1 is upregulated in polycystic ovary syndrome (pcos) and suppresses the role of microrna-19b in the proliferation of ovarian granulose cells: short title: bbox1 antisense rna 1 in cell proliferation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512487/
https://www.ncbi.nlm.nih.gov/pubmed/37735639
http://dx.doi.org/10.1186/s12905-023-02632-5
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