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Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)

Serum protein electrophoresis has been demonstrated to have utility in diagnostic workup, wellness exams, and prognosis. Agarose gel electrophoresis (AGE) has previously been described for use with serum from Asian elephants (Elephas maximus). As the newer method of capillary zone electrophoresis (C...

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Autores principales: Osorio, Shirley, Soto, Jeny, Schmitt, Dennis, Kiso, Wendy, Cray, Carolyn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512825/
https://www.ncbi.nlm.nih.gov/pubmed/37745210
http://dx.doi.org/10.3389/fvets.2023.1204880
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author Osorio, Shirley
Soto, Jeny
Schmitt, Dennis
Kiso, Wendy
Cray, Carolyn
author_facet Osorio, Shirley
Soto, Jeny
Schmitt, Dennis
Kiso, Wendy
Cray, Carolyn
author_sort Osorio, Shirley
collection PubMed
description Serum protein electrophoresis has been demonstrated to have utility in diagnostic workup, wellness exams, and prognosis. Agarose gel electrophoresis (AGE) has previously been described for use with serum from Asian elephants (Elephas maximus). As the newer method of capillary zone electrophoresis (CZE) is becoming more commonplace in veterinary diagnostic laboratories, serum samples from Asian elephants were examined using this method. CZE allowed for a reproducible definition of two beta fractions and, overall, showed a low coefficient of variation for fraction quantitation. Preliminary reference intervals were generated using samples primarily from an older population of 22 female elephants. Albumin levels determined by CZE were also compared with those determined by the bromocresol green method on a chemistry analyzer. It was found that the latter method overestimated the level of albumin with a mean positive bias of 11.6% or 0.38 g/dL, thus method-specific reference intervals should be used. Significant negative correlations were observed between A/G ratio determined by CZE and serum amyloid A levels (r = −0.47, p < 0.0001) and haptoglobin (r = −0.52, p < 0.0001); both APP were significantly correlated with the alpha 2 globulin fraction (p < 0.0001). CZE reflects an overall picture of changes in acute phase proteins and immunoglobulins and accurate quantitation of albumin and thus should be considered as an adjunct tool to the use of other measures of the acute phase response in patient monitoring.
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spelling pubmed-105128252023-09-22 Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus) Osorio, Shirley Soto, Jeny Schmitt, Dennis Kiso, Wendy Cray, Carolyn Front Vet Sci Veterinary Science Serum protein electrophoresis has been demonstrated to have utility in diagnostic workup, wellness exams, and prognosis. Agarose gel electrophoresis (AGE) has previously been described for use with serum from Asian elephants (Elephas maximus). As the newer method of capillary zone electrophoresis (CZE) is becoming more commonplace in veterinary diagnostic laboratories, serum samples from Asian elephants were examined using this method. CZE allowed for a reproducible definition of two beta fractions and, overall, showed a low coefficient of variation for fraction quantitation. Preliminary reference intervals were generated using samples primarily from an older population of 22 female elephants. Albumin levels determined by CZE were also compared with those determined by the bromocresol green method on a chemistry analyzer. It was found that the latter method overestimated the level of albumin with a mean positive bias of 11.6% or 0.38 g/dL, thus method-specific reference intervals should be used. Significant negative correlations were observed between A/G ratio determined by CZE and serum amyloid A levels (r = −0.47, p < 0.0001) and haptoglobin (r = −0.52, p < 0.0001); both APP were significantly correlated with the alpha 2 globulin fraction (p < 0.0001). CZE reflects an overall picture of changes in acute phase proteins and immunoglobulins and accurate quantitation of albumin and thus should be considered as an adjunct tool to the use of other measures of the acute phase response in patient monitoring. Frontiers Media S.A. 2023-09-07 /pmc/articles/PMC10512825/ /pubmed/37745210 http://dx.doi.org/10.3389/fvets.2023.1204880 Text en Copyright © 2023 Osorio, Soto, Schmitt, Kiso and Cray. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Osorio, Shirley
Soto, Jeny
Schmitt, Dennis
Kiso, Wendy
Cray, Carolyn
Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title_full Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title_fullStr Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title_full_unstemmed Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title_short Preliminary assessment of serum capillary zone electrophoresis in the Asian elephant (Elephas maximus)
title_sort preliminary assessment of serum capillary zone electrophoresis in the asian elephant (elephas maximus)
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512825/
https://www.ncbi.nlm.nih.gov/pubmed/37745210
http://dx.doi.org/10.3389/fvets.2023.1204880
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