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Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes

BACKGROUND: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and fibromyalgia have overlapping neurologic symptoms particularly disabling fatigue. This has given rise to the question whether they are distinct central nervous system (CNS) entities or is one an extension of the other. MATER...

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Autores principales: Schutzer, Steven E., Liu, Tao, Tsai, Chia-Feng, Petyuk, Vladislav A., Schepmoes, Athena A., Wang, Yi-Ting, Weitz, Karl K., Bergquist, Jonas, Smith, Richard D., Natelson, Benjamin H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512920/
https://www.ncbi.nlm.nih.gov/pubmed/37722890
http://dx.doi.org/10.1080/07853890.2023.2208372
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author Schutzer, Steven E.
Liu, Tao
Tsai, Chia-Feng
Petyuk, Vladislav A.
Schepmoes, Athena A.
Wang, Yi-Ting
Weitz, Karl K.
Bergquist, Jonas
Smith, Richard D.
Natelson, Benjamin H.
author_facet Schutzer, Steven E.
Liu, Tao
Tsai, Chia-Feng
Petyuk, Vladislav A.
Schepmoes, Athena A.
Wang, Yi-Ting
Weitz, Karl K.
Bergquist, Jonas
Smith, Richard D.
Natelson, Benjamin H.
author_sort Schutzer, Steven E.
collection PubMed
description BACKGROUND: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and fibromyalgia have overlapping neurologic symptoms particularly disabling fatigue. This has given rise to the question whether they are distinct central nervous system (CNS) entities or is one an extension of the other. MATERIAL AND METHODS: To investigate this, we used unbiased quantitative mass spectrometry-based proteomics to examine the most proximal fluid to the brain, cerebrospinal fluid (CSF). This was to ascertain if the proteome profile of one was the same or different from the other. We examined two separate groups of ME/CFS, one with (n = 15) and one without (n = 15) fibromyalgia. RESULTS: We quantified a total of 2083 proteins using immunoaffinity depletion, tandem mass tag isobaric labelling and offline two-dimensional liquid chromatography coupled to tandem mass spectrometry, including 1789 that were quantified in all the CSF samples. ANOVA analysis did not yield any proteins with an adjusted p value <.05. CONCLUSION: This supports the notion that ME/CFS and fibromyalgia as currently defined are not distinct entities. KEY MESSAGE: 1. ME/CFS and fibromyalgia as currently defined are not distinct entities. 2. Unbiased quantitative mass spectrometry-based proteomics can be used to discover cerebrospinal fluid proteins that are biomarkers for a condition such as we are studying.
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spelling pubmed-105129202023-09-22 Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes Schutzer, Steven E. Liu, Tao Tsai, Chia-Feng Petyuk, Vladislav A. Schepmoes, Athena A. Wang, Yi-Ting Weitz, Karl K. Bergquist, Jonas Smith, Richard D. Natelson, Benjamin H. Ann Med Neurology BACKGROUND: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and fibromyalgia have overlapping neurologic symptoms particularly disabling fatigue. This has given rise to the question whether they are distinct central nervous system (CNS) entities or is one an extension of the other. MATERIAL AND METHODS: To investigate this, we used unbiased quantitative mass spectrometry-based proteomics to examine the most proximal fluid to the brain, cerebrospinal fluid (CSF). This was to ascertain if the proteome profile of one was the same or different from the other. We examined two separate groups of ME/CFS, one with (n = 15) and one without (n = 15) fibromyalgia. RESULTS: We quantified a total of 2083 proteins using immunoaffinity depletion, tandem mass tag isobaric labelling and offline two-dimensional liquid chromatography coupled to tandem mass spectrometry, including 1789 that were quantified in all the CSF samples. ANOVA analysis did not yield any proteins with an adjusted p value <.05. CONCLUSION: This supports the notion that ME/CFS and fibromyalgia as currently defined are not distinct entities. KEY MESSAGE: 1. ME/CFS and fibromyalgia as currently defined are not distinct entities. 2. Unbiased quantitative mass spectrometry-based proteomics can be used to discover cerebrospinal fluid proteins that are biomarkers for a condition such as we are studying. Taylor & Francis 2023-09-18 /pmc/articles/PMC10512920/ /pubmed/37722890 http://dx.doi.org/10.1080/07853890.2023.2208372 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
spellingShingle Neurology
Schutzer, Steven E.
Liu, Tao
Tsai, Chia-Feng
Petyuk, Vladislav A.
Schepmoes, Athena A.
Wang, Yi-Ting
Weitz, Karl K.
Bergquist, Jonas
Smith, Richard D.
Natelson, Benjamin H.
Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title_full Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title_fullStr Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title_full_unstemmed Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title_short Myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
title_sort myalgic encephalomyelitis/chronic fatigue syndrome and fibromyalgia are indistinguishable by their cerebrospinal fluid proteomes
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10512920/
https://www.ncbi.nlm.nih.gov/pubmed/37722890
http://dx.doi.org/10.1080/07853890.2023.2208372
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