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Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513271/ https://www.ncbi.nlm.nih.gov/pubmed/37733779 http://dx.doi.org/10.1371/journal.pone.0290875 |
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author | Härer, Andreas Rennison, Diana J. |
author_facet | Härer, Andreas Rennison, Diana J. |
author_sort | Härer, Andreas |
collection | PubMed |
description | The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities, as estimated from lethal sampling of intestinal tissue. We further lack knowledge on whether non-lethal sampling methods are suitable for detecting gut microbiota shifts associated with changes in environmental factors (e.g., diet). We addressed these questions in threespine stickleback fish, a model system for evolutionary ecology, by comparing bacterial communities from intestinal tissue and feces. Despite some differences in community composition between the two sample types and considerable temporal variation among fecal samples, bacterial communities appear to largely overlap. Further, we detected consistent and significant changes of fecal bacterial communities associated with an experimental diet manipulation. This suggests that fecal sampling can represent an adequate non-lethal method to characterize the gut microbiota of threespine stickleback, but additional studies will be necessary before drawing general conclusions regarding the validity of fecal sampling for gut microbiota studies. To this end, we give recommendations to improve the characterization of the gut microbiota via fecal sampling. Fecal sampling allows studying temporal gut microbiota shifts associated with environmental change at the individual level, which increases opportunities for future experimental gut microbiota research. |
format | Online Article Text |
id | pubmed-10513271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105132712023-09-22 Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota Härer, Andreas Rennison, Diana J. PLoS One Research Article The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities, as estimated from lethal sampling of intestinal tissue. We further lack knowledge on whether non-lethal sampling methods are suitable for detecting gut microbiota shifts associated with changes in environmental factors (e.g., diet). We addressed these questions in threespine stickleback fish, a model system for evolutionary ecology, by comparing bacterial communities from intestinal tissue and feces. Despite some differences in community composition between the two sample types and considerable temporal variation among fecal samples, bacterial communities appear to largely overlap. Further, we detected consistent and significant changes of fecal bacterial communities associated with an experimental diet manipulation. This suggests that fecal sampling can represent an adequate non-lethal method to characterize the gut microbiota of threespine stickleback, but additional studies will be necessary before drawing general conclusions regarding the validity of fecal sampling for gut microbiota studies. To this end, we give recommendations to improve the characterization of the gut microbiota via fecal sampling. Fecal sampling allows studying temporal gut microbiota shifts associated with environmental change at the individual level, which increases opportunities for future experimental gut microbiota research. Public Library of Science 2023-09-21 /pmc/articles/PMC10513271/ /pubmed/37733779 http://dx.doi.org/10.1371/journal.pone.0290875 Text en © 2023 Härer, Rennison https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Härer, Andreas Rennison, Diana J. Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title | Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title_full | Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title_fullStr | Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title_full_unstemmed | Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title_short | Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
title_sort | assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513271/ https://www.ncbi.nlm.nih.gov/pubmed/37733779 http://dx.doi.org/10.1371/journal.pone.0290875 |
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