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Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota

The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities,...

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Autores principales: Härer, Andreas, Rennison, Diana J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513271/
https://www.ncbi.nlm.nih.gov/pubmed/37733779
http://dx.doi.org/10.1371/journal.pone.0290875
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author Härer, Andreas
Rennison, Diana J.
author_facet Härer, Andreas
Rennison, Diana J.
author_sort Härer, Andreas
collection PubMed
description The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities, as estimated from lethal sampling of intestinal tissue. We further lack knowledge on whether non-lethal sampling methods are suitable for detecting gut microbiota shifts associated with changes in environmental factors (e.g., diet). We addressed these questions in threespine stickleback fish, a model system for evolutionary ecology, by comparing bacterial communities from intestinal tissue and feces. Despite some differences in community composition between the two sample types and considerable temporal variation among fecal samples, bacterial communities appear to largely overlap. Further, we detected consistent and significant changes of fecal bacterial communities associated with an experimental diet manipulation. This suggests that fecal sampling can represent an adequate non-lethal method to characterize the gut microbiota of threespine stickleback, but additional studies will be necessary before drawing general conclusions regarding the validity of fecal sampling for gut microbiota studies. To this end, we give recommendations to improve the characterization of the gut microbiota via fecal sampling. Fecal sampling allows studying temporal gut microbiota shifts associated with environmental change at the individual level, which increases opportunities for future experimental gut microbiota research.
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spelling pubmed-105132712023-09-22 Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota Härer, Andreas Rennison, Diana J. PLoS One Research Article The gut microbiota is crucial for many aspects of their hosts’ biology, and it has been characterized for many species across the animal kingdom. Yet, we still don’t have a good understanding of whether non-lethal sampling can accurately capture the diversity of gut-associated bacterial communities, as estimated from lethal sampling of intestinal tissue. We further lack knowledge on whether non-lethal sampling methods are suitable for detecting gut microbiota shifts associated with changes in environmental factors (e.g., diet). We addressed these questions in threespine stickleback fish, a model system for evolutionary ecology, by comparing bacterial communities from intestinal tissue and feces. Despite some differences in community composition between the two sample types and considerable temporal variation among fecal samples, bacterial communities appear to largely overlap. Further, we detected consistent and significant changes of fecal bacterial communities associated with an experimental diet manipulation. This suggests that fecal sampling can represent an adequate non-lethal method to characterize the gut microbiota of threespine stickleback, but additional studies will be necessary before drawing general conclusions regarding the validity of fecal sampling for gut microbiota studies. To this end, we give recommendations to improve the characterization of the gut microbiota via fecal sampling. Fecal sampling allows studying temporal gut microbiota shifts associated with environmental change at the individual level, which increases opportunities for future experimental gut microbiota research. Public Library of Science 2023-09-21 /pmc/articles/PMC10513271/ /pubmed/37733779 http://dx.doi.org/10.1371/journal.pone.0290875 Text en © 2023 Härer, Rennison https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Härer, Andreas
Rennison, Diana J.
Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title_full Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title_fullStr Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title_full_unstemmed Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title_short Assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
title_sort assessing the validity of fecal sampling for characterizing variation in threespine stickleback’s gut microbiota
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513271/
https://www.ncbi.nlm.nih.gov/pubmed/37733779
http://dx.doi.org/10.1371/journal.pone.0290875
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