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Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks
The stable isotope technique provides the possibility to trace ancient textiles because the technique is associated with advantages such as trace indication, fast detection, and accurate results. Since different cocooning conditions may impact cocoons even under identical habitats, it is important t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513321/ https://www.ncbi.nlm.nih.gov/pubmed/37733796 http://dx.doi.org/10.1371/journal.pone.0291769 |
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author | Li, Hao He, Yujie Jia, Liling Liu, Yong Yang, Dan Shao, Shuai Lv, Gang Yang, Hailiang Zheng, Hailing Cui, Xuhong Zhou, Yang Peng, Zhiqin |
author_facet | Li, Hao He, Yujie Jia, Liling Liu, Yong Yang, Dan Shao, Shuai Lv, Gang Yang, Hailiang Zheng, Hailing Cui, Xuhong Zhou, Yang Peng, Zhiqin |
author_sort | Li, Hao |
collection | PubMed |
description | The stable isotope technique provides the possibility to trace ancient textiles because the technique is associated with advantages such as trace indication, fast detection, and accurate results. Since different cocooning conditions may impact cocoons even under identical habitats, it is important to investigate the effects of different cocooning temperatures and humidity on the isotope incorporation values in the cocoons. In this study, silk fibers were reeled under different conditions of temperature and humidity, followed by analysis of the secondary structure of cocoon proteins and isotope incorporation patterns. We found that the deviations in carbon isotope values of silk under different cocooning conditions could reach up to 0.76‰, while the deviation in carbon isotope values at different locations of a single silk was 2.75‰. Further, during the cocooning process, depletion of the (13)C-isotope at different locations of the silk fibers was observed, reducing the δ(13)C values. We proposed that the changes in carbon isotopes in silk were related to the content of sericin and silk fibroin in silk. Finally, we did not observe a significant difference in isotope ratios in degummed cocoons. In summary, the (13)C isotope was enriched in sericin, whereas (15)N was enriched in fibroin, and these findings provide basic information for tracing the provenance of silks. |
format | Online Article Text |
id | pubmed-10513321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-105133212023-09-22 Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks Li, Hao He, Yujie Jia, Liling Liu, Yong Yang, Dan Shao, Shuai Lv, Gang Yang, Hailiang Zheng, Hailing Cui, Xuhong Zhou, Yang Peng, Zhiqin PLoS One Research Article The stable isotope technique provides the possibility to trace ancient textiles because the technique is associated with advantages such as trace indication, fast detection, and accurate results. Since different cocooning conditions may impact cocoons even under identical habitats, it is important to investigate the effects of different cocooning temperatures and humidity on the isotope incorporation values in the cocoons. In this study, silk fibers were reeled under different conditions of temperature and humidity, followed by analysis of the secondary structure of cocoon proteins and isotope incorporation patterns. We found that the deviations in carbon isotope values of silk under different cocooning conditions could reach up to 0.76‰, while the deviation in carbon isotope values at different locations of a single silk was 2.75‰. Further, during the cocooning process, depletion of the (13)C-isotope at different locations of the silk fibers was observed, reducing the δ(13)C values. We proposed that the changes in carbon isotopes in silk were related to the content of sericin and silk fibroin in silk. Finally, we did not observe a significant difference in isotope ratios in degummed cocoons. In summary, the (13)C isotope was enriched in sericin, whereas (15)N was enriched in fibroin, and these findings provide basic information for tracing the provenance of silks. Public Library of Science 2023-09-21 /pmc/articles/PMC10513321/ /pubmed/37733796 http://dx.doi.org/10.1371/journal.pone.0291769 Text en © 2023 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Li, Hao He, Yujie Jia, Liling Liu, Yong Yang, Dan Shao, Shuai Lv, Gang Yang, Hailiang Zheng, Hailing Cui, Xuhong Zhou, Yang Peng, Zhiqin Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title | Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title_full | Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title_fullStr | Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title_full_unstemmed | Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title_short | Effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
title_sort | effect of cocooning conditions on the structure, carbon and nitrogen isotope ratios of silks |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513321/ https://www.ncbi.nlm.nih.gov/pubmed/37733796 http://dx.doi.org/10.1371/journal.pone.0291769 |
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