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Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases

BACKGROUND: Systemic inflammation may cause endothelial activation, mediate local inflammation, and accelerate progression of atherosclerosis. We examined whether the levels of circulating inflammatory cytokines reflect local vascular inflammation and oxidative stress in two types of human arteries....

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Autores principales: Sulicka-Grodzicka, Joanna, Szczepaniak, Piotr, Jozefczuk, Ewelina, Urbanski, Karol, Siedlinski, Mateusz, Niewiara, Łukasz, Guzik, Bartłomiej, Filip, Grzegorz, Kapelak, Bogusław, Wierzbicki, Karol, Korkosz, Mariusz, Guzik, Tomasz J., Mikolajczyk, Tomasz P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513373/
https://www.ncbi.nlm.nih.gov/pubmed/37745103
http://dx.doi.org/10.3389/fcvm.2023.1230051
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author Sulicka-Grodzicka, Joanna
Szczepaniak, Piotr
Jozefczuk, Ewelina
Urbanski, Karol
Siedlinski, Mateusz
Niewiara, Łukasz
Guzik, Bartłomiej
Filip, Grzegorz
Kapelak, Bogusław
Wierzbicki, Karol
Korkosz, Mariusz
Guzik, Tomasz J.
Mikolajczyk, Tomasz P.
author_facet Sulicka-Grodzicka, Joanna
Szczepaniak, Piotr
Jozefczuk, Ewelina
Urbanski, Karol
Siedlinski, Mateusz
Niewiara, Łukasz
Guzik, Bartłomiej
Filip, Grzegorz
Kapelak, Bogusław
Wierzbicki, Karol
Korkosz, Mariusz
Guzik, Tomasz J.
Mikolajczyk, Tomasz P.
author_sort Sulicka-Grodzicka, Joanna
collection PubMed
description BACKGROUND: Systemic inflammation may cause endothelial activation, mediate local inflammation, and accelerate progression of atherosclerosis. We examined whether the levels of circulating inflammatory cytokines reflect local vascular inflammation and oxidative stress in two types of human arteries. METHODS: Human internal mammary artery (IMA) was obtained in 69 patients undergoing coronary artery bypass graft (CABG) surgery and left anterior descending (LAD) artery was obtained in 17 patients undergoing heart transplantation (HTx). Plasma levels of tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were measured using ELISA, high-sensitivity C-reactive protein (hs-CRP) was measured using Luminex, and mRNA expression of proinflammatory cytokines in the vascular tissues was assessed. Furthermore, formation of superoxide anion was measured in segments of IMA using 5 uM lucigenin-dependent chemiluminescence. Vascular reactivity was measured using tissue organ bath system. RESULTS: TNF-α, IL-6 and IL-1β mRNAs were expressed in all studied IMA and LAD segments. Plasma levels of inflammatory cytokines did not correlate with vascular cytokine mRNA expression neither in IMA nor in LAD. Plasma TNF-α and IL-6 correlated with hs-CRP level in CABG group. Hs-CRP also correlated with TNF-α in HTx group. Neither vascular TNF-α, IL-6 and IL-1β mRNA expression, nor systemic levels of either TNF-α, IL-6 and IL-1β were correlated with superoxide generation in IMAs. Interestingly, circulating IL-1β negatively correlated with maximal relaxation of the internal mammary artery (r = −0.37, p = 0.004). At the same time the mRNA expression of studied inflammatory cytokines were positively associated with each other in both IMA and LAD. The positive correlations were observed between circulating levels of IL-6 and TNF-α in CABG cohort and IL-6 and IL-1β in HTx cohort. CONCLUSIONS: This study shows that peripheral inflammatory cytokine measurements may not reflect local vascular inflammation or oxidative stress in patients with advanced cardiovascular disease (CVD). Circulating pro-inflammatory cytokines generally correlated positively with each other, similarly their mRNA correlated in the arterial wall, however, these levels were not correlated between the studied compartments.
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spelling pubmed-105133732023-09-22 Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases Sulicka-Grodzicka, Joanna Szczepaniak, Piotr Jozefczuk, Ewelina Urbanski, Karol Siedlinski, Mateusz Niewiara, Łukasz Guzik, Bartłomiej Filip, Grzegorz Kapelak, Bogusław Wierzbicki, Karol Korkosz, Mariusz Guzik, Tomasz J. Mikolajczyk, Tomasz P. Front Cardiovasc Med Cardiovascular Medicine BACKGROUND: Systemic inflammation may cause endothelial activation, mediate local inflammation, and accelerate progression of atherosclerosis. We examined whether the levels of circulating inflammatory cytokines reflect local vascular inflammation and oxidative stress in two types of human arteries. METHODS: Human internal mammary artery (IMA) was obtained in 69 patients undergoing coronary artery bypass graft (CABG) surgery and left anterior descending (LAD) artery was obtained in 17 patients undergoing heart transplantation (HTx). Plasma levels of tumor necrosis factor α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) were measured using ELISA, high-sensitivity C-reactive protein (hs-CRP) was measured using Luminex, and mRNA expression of proinflammatory cytokines in the vascular tissues was assessed. Furthermore, formation of superoxide anion was measured in segments of IMA using 5 uM lucigenin-dependent chemiluminescence. Vascular reactivity was measured using tissue organ bath system. RESULTS: TNF-α, IL-6 and IL-1β mRNAs were expressed in all studied IMA and LAD segments. Plasma levels of inflammatory cytokines did not correlate with vascular cytokine mRNA expression neither in IMA nor in LAD. Plasma TNF-α and IL-6 correlated with hs-CRP level in CABG group. Hs-CRP also correlated with TNF-α in HTx group. Neither vascular TNF-α, IL-6 and IL-1β mRNA expression, nor systemic levels of either TNF-α, IL-6 and IL-1β were correlated with superoxide generation in IMAs. Interestingly, circulating IL-1β negatively correlated with maximal relaxation of the internal mammary artery (r = −0.37, p = 0.004). At the same time the mRNA expression of studied inflammatory cytokines were positively associated with each other in both IMA and LAD. The positive correlations were observed between circulating levels of IL-6 and TNF-α in CABG cohort and IL-6 and IL-1β in HTx cohort. CONCLUSIONS: This study shows that peripheral inflammatory cytokine measurements may not reflect local vascular inflammation or oxidative stress in patients with advanced cardiovascular disease (CVD). Circulating pro-inflammatory cytokines generally correlated positively with each other, similarly their mRNA correlated in the arterial wall, however, these levels were not correlated between the studied compartments. Frontiers Media S.A. 2023-09-07 /pmc/articles/PMC10513373/ /pubmed/37745103 http://dx.doi.org/10.3389/fcvm.2023.1230051 Text en © 2023 Sulicka-Grodzicka, Szczepaniak, Jozefczuk, Urbanski, Siedlinski, Niewiara, Guzik, Filip, Kapelak, Wierzbicki, Korkosz, Guzik and Mikolajczyk. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Sulicka-Grodzicka, Joanna
Szczepaniak, Piotr
Jozefczuk, Ewelina
Urbanski, Karol
Siedlinski, Mateusz
Niewiara, Łukasz
Guzik, Bartłomiej
Filip, Grzegorz
Kapelak, Bogusław
Wierzbicki, Karol
Korkosz, Mariusz
Guzik, Tomasz J.
Mikolajczyk, Tomasz P.
Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title_full Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title_fullStr Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title_full_unstemmed Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title_short Systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
title_sort systemic and local vascular inflammation and arterial reactive oxygen species generation in patients with advanced cardiovascular diseases
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513373/
https://www.ncbi.nlm.nih.gov/pubmed/37745103
http://dx.doi.org/10.3389/fcvm.2023.1230051
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