Cargando…

Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment

Total saponins from Trillium tschonoskii Maxim (TSTT), a bioactive component of local natural herbs in the Enshi area, China, have been demonstrated to have functions of restoring cognitive capacity and promoting axonal regeneration post-stroke, but the mechanism of this process remains unclear. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Gang, Tang, Xiane, Zhao, Fangyu, Qin, Xiaoli, Wang, Fengjie, Yang, Dan, Zhu, Hong, Chen, Xianbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513410/
https://www.ncbi.nlm.nih.gov/pubmed/37745057
http://dx.doi.org/10.3389/fphar.2023.1255560
_version_ 1785108564022394880
author Wang, Gang
Tang, Xiane
Zhao, Fangyu
Qin, Xiaoli
Wang, Fengjie
Yang, Dan
Zhu, Hong
Chen, Xianbing
author_facet Wang, Gang
Tang, Xiane
Zhao, Fangyu
Qin, Xiaoli
Wang, Fengjie
Yang, Dan
Zhu, Hong
Chen, Xianbing
author_sort Wang, Gang
collection PubMed
description Total saponins from Trillium tschonoskii Maxim (TSTT), a bioactive component of local natural herbs in the Enshi area, China, have been demonstrated to have functions of restoring cognitive capacity and promoting axonal regeneration post-stroke, but the mechanism of this process remains unclear. The hippocampus is a critical tissue for controlling learning and memory capacity, and the sonic hedgehog (Shh) signaling pathway plays a major role in the patterning and synaptic plasticity of hippocampal neural circuits. Therefore, we aimed to investigate whether TSTT could restore learning and cognitive functions by modulating the Shh pathway in rats with post-stroke cognitive impairment (PSCI). The ischemia model was established by permanent middle cerebral artery occlusion (MCAO) in 100 Sprague–Dawley (SD) rats, and the model rats were administered using TSTT (100 mg/kg) or donepezil hydrochloride as the positive control (daily 0.45 mg/kg, DON) for 4 weeks after the operation. As assessed by the Morris water maze test, the cognitive function of PSCI rats was significantly improved upon TSTT treatment. Meanwhile, the cerebral infarct volume reduced with TSTT, as shown by HE and TTC staining, and the number of Nissl bodies and dendritic spine density were significantly increased, as shown by Nissl and Golgi staining. In addition, TSTT upregulated PSD-95, SYN, and GAP-43, and inhibited neuronal apoptosis, as evidenced by increased Bcl-2 levels along with decreased Bax and caspase-3 expression. TSTT could also significantly upregulate Shh, Ptch1, Smo, and Gli1 proteins, indicating the activation of the Shh signaling pathway. Therefore, TSTT can protect PSCI rats by inhibiting apoptosis and promoting neuronal synaptic remodeling. The Shh pathway is also involved.
format Online
Article
Text
id pubmed-10513410
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105134102023-09-22 Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment Wang, Gang Tang, Xiane Zhao, Fangyu Qin, Xiaoli Wang, Fengjie Yang, Dan Zhu, Hong Chen, Xianbing Front Pharmacol Pharmacology Total saponins from Trillium tschonoskii Maxim (TSTT), a bioactive component of local natural herbs in the Enshi area, China, have been demonstrated to have functions of restoring cognitive capacity and promoting axonal regeneration post-stroke, but the mechanism of this process remains unclear. The hippocampus is a critical tissue for controlling learning and memory capacity, and the sonic hedgehog (Shh) signaling pathway plays a major role in the patterning and synaptic plasticity of hippocampal neural circuits. Therefore, we aimed to investigate whether TSTT could restore learning and cognitive functions by modulating the Shh pathway in rats with post-stroke cognitive impairment (PSCI). The ischemia model was established by permanent middle cerebral artery occlusion (MCAO) in 100 Sprague–Dawley (SD) rats, and the model rats were administered using TSTT (100 mg/kg) or donepezil hydrochloride as the positive control (daily 0.45 mg/kg, DON) for 4 weeks after the operation. As assessed by the Morris water maze test, the cognitive function of PSCI rats was significantly improved upon TSTT treatment. Meanwhile, the cerebral infarct volume reduced with TSTT, as shown by HE and TTC staining, and the number of Nissl bodies and dendritic spine density were significantly increased, as shown by Nissl and Golgi staining. In addition, TSTT upregulated PSD-95, SYN, and GAP-43, and inhibited neuronal apoptosis, as evidenced by increased Bcl-2 levels along with decreased Bax and caspase-3 expression. TSTT could also significantly upregulate Shh, Ptch1, Smo, and Gli1 proteins, indicating the activation of the Shh signaling pathway. Therefore, TSTT can protect PSCI rats by inhibiting apoptosis and promoting neuronal synaptic remodeling. The Shh pathway is also involved. Frontiers Media S.A. 2023-09-07 /pmc/articles/PMC10513410/ /pubmed/37745057 http://dx.doi.org/10.3389/fphar.2023.1255560 Text en Copyright © 2023 Wang, Tang, Zhao, Qin, Wang, Yang, Zhu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Gang
Tang, Xiane
Zhao, Fangyu
Qin, Xiaoli
Wang, Fengjie
Yang, Dan
Zhu, Hong
Chen, Xianbing
Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title_full Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title_fullStr Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title_full_unstemmed Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title_short Total saponins from Trillium tschonoskii Maxim promote neurological recovery in model rats with post-stroke cognitive impairment
title_sort total saponins from trillium tschonoskii maxim promote neurological recovery in model rats with post-stroke cognitive impairment
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513410/
https://www.ncbi.nlm.nih.gov/pubmed/37745057
http://dx.doi.org/10.3389/fphar.2023.1255560
work_keys_str_mv AT wanggang totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT tangxiane totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT zhaofangyu totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT qinxiaoli totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT wangfengjie totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT yangdan totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT zhuhong totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment
AT chenxianbing totalsaponinsfromtrilliumtschonoskiimaximpromoteneurologicalrecoveryinmodelratswithpoststrokecognitiveimpairment