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Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways

Light serves not only as a signaling cue perceived by plant photoreceptors but also as an essential energy source captured by chloroplasts. However, excessive light can impose stress on plants. Fern gametophytes possess the unique ability to survive independently and play a critical role in the alte...

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Autores principales: Cai, Zeping, Wang, Xiaochen, Xie, Zhenyu, Wen, Zhenyi, Yu, Xudong, Xu, Shitao, Su, Xinyu, Luo, Jiajia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513451/
https://www.ncbi.nlm.nih.gov/pubmed/37746005
http://dx.doi.org/10.3389/fpls.2023.1222414
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author Cai, Zeping
Wang, Xiaochen
Xie, Zhenyu
Wen, Zhenyi
Yu, Xudong
Xu, Shitao
Su, Xinyu
Luo, Jiajia
author_facet Cai, Zeping
Wang, Xiaochen
Xie, Zhenyu
Wen, Zhenyi
Yu, Xudong
Xu, Shitao
Su, Xinyu
Luo, Jiajia
author_sort Cai, Zeping
collection PubMed
description Light serves not only as a signaling cue perceived by plant photoreceptors but also as an essential energy source captured by chloroplasts. However, excessive light can impose stress on plants. Fern gametophytes possess the unique ability to survive independently and play a critical role in the alternation of generations. Due to their predominantly shaded distribution under canopies, light availability becomes a limiting factor for gametophyte survival, making it imperative to investigate their response to light. Previous research on fern gametophytes’ light response has been limited to the physiological level. In this study, we examined the light response of Adiantum flabellulatum gametophytes under different photosynthetic photon flux density (PPFD) levels and identified their high sensitivity to low light. We thereby determined optimal and stress-inducing light conditions. By employing transcriptome sequencing, weighted gene co-expression network analysis, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, we identified 10,995 differentially expressed genes (DEGs). Notably, 3 PHYBs and 5 Type 1 CRYs (CRY1s) were significantly down-regulated at low PPFD (0.1 μmol m(-2) s(-1)). Furthermore, we annotated 927 DEGs to pathways related to photosynthesis and 210 to the flavonoid biosynthesis pathway involved in photoprotection. Additionally, we predicted 34 transcription factor families and identified a close correlation between mTERFs and photosynthesis, as well as a strong co-expression relationship between MYBs and bHLHs and genes encoding flavonoid synthesis enzymes. This comprehensive analysis enhances our understanding of the light response of fern gametophytes and provides novel insights into the mechanisms governing their responses to light.
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spelling pubmed-105134512023-09-22 Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways Cai, Zeping Wang, Xiaochen Xie, Zhenyu Wen, Zhenyi Yu, Xudong Xu, Shitao Su, Xinyu Luo, Jiajia Front Plant Sci Plant Science Light serves not only as a signaling cue perceived by plant photoreceptors but also as an essential energy source captured by chloroplasts. However, excessive light can impose stress on plants. Fern gametophytes possess the unique ability to survive independently and play a critical role in the alternation of generations. Due to their predominantly shaded distribution under canopies, light availability becomes a limiting factor for gametophyte survival, making it imperative to investigate their response to light. Previous research on fern gametophytes’ light response has been limited to the physiological level. In this study, we examined the light response of Adiantum flabellulatum gametophytes under different photosynthetic photon flux density (PPFD) levels and identified their high sensitivity to low light. We thereby determined optimal and stress-inducing light conditions. By employing transcriptome sequencing, weighted gene co-expression network analysis, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses, we identified 10,995 differentially expressed genes (DEGs). Notably, 3 PHYBs and 5 Type 1 CRYs (CRY1s) were significantly down-regulated at low PPFD (0.1 μmol m(-2) s(-1)). Furthermore, we annotated 927 DEGs to pathways related to photosynthesis and 210 to the flavonoid biosynthesis pathway involved in photoprotection. Additionally, we predicted 34 transcription factor families and identified a close correlation between mTERFs and photosynthesis, as well as a strong co-expression relationship between MYBs and bHLHs and genes encoding flavonoid synthesis enzymes. This comprehensive analysis enhances our understanding of the light response of fern gametophytes and provides novel insights into the mechanisms governing their responses to light. Frontiers Media S.A. 2023-09-07 /pmc/articles/PMC10513451/ /pubmed/37746005 http://dx.doi.org/10.3389/fpls.2023.1222414 Text en Copyright © 2023 Cai, Wang, Xie, Wen, Yu, Xu, Su and Luo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Cai, Zeping
Wang, Xiaochen
Xie, Zhenyu
Wen, Zhenyi
Yu, Xudong
Xu, Shitao
Su, Xinyu
Luo, Jiajia
Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title_full Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title_fullStr Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title_full_unstemmed Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title_short Light response of gametophyte in Adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
title_sort light response of gametophyte in adiantum flabellulatum: transcriptome analysis and identification of key genes and pathways
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10513451/
https://www.ncbi.nlm.nih.gov/pubmed/37746005
http://dx.doi.org/10.3389/fpls.2023.1222414
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